Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Mouse Testis Tissue.)
Rabbit anti-Mouse Zuotin Related Factor 1 Polyclonal Antibody | anti-ZRF1 antibody
Zuotin Related Factor 1 (ZRF1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Mouse Testis Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Mouse Testis Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ZRF1, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ZRF1, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)
NCBI and Uniprot Product Information
Uniprot Description
DNAJC2: Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Chaperone; DNA replication
Chromosomal Location of Human Ortholog: 5 A3|5 9.97 cM
Cellular Component: cytoplasm; cytosol; nuclear membrane; nucleus
Molecular Function: chromatin binding; DNA binding; histone binding; Hsp70 protein binding; protein binding; RNA binding
Biological Process: covalent chromatin modification; DNA replication; negative regulation of cell growth; positive regulation of transcription, DNA-templated; regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on ZRF1
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Product Notes
The ZRF1 dnajc2 (Catalog #AAA2032297) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Zuotin Related Factor 1 (ZRF1) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Zuotin Related Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ZRF1 dnajc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zuotin Related Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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