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Immunohistochemistry (IHC) (Anti-GCNT3 antibody IHC of human stomach. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Rabbit GCNT3 Polyclonal Antibody | anti-GCNT3 antibody

Rabbit Polyclonal to Human GCNT3

Gene Names
GCNT3; GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
Reactivity
Gibbon, Dog, Gorilla, Horse, Human
Predicted Reactivity: Monkey, Bovine, Pig, Sheep (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
GCNT3; Polyclonal Antibody; Rabbit Polyclonal to Human GCNT3; Anti-GCNT3 Antibody (Internal) IHC-plus; Beta1;6GlcNAc-transferase; C24GNT; C2/4GnT; C2GnT-mucin type; C2GNTM; C2GnT-M; C2GNT2; GNTM; HC2GnT-M; Human GCNT3; anti-GCNT3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Gibbon, Dog, Gorilla, Horse, Human
Predicted Reactivity: Monkey, Bovine, Pig, Sheep (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Human GCNT3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.1% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
438
Applicable Applications for anti-GCNT3 antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes
IHC-P (5 - 7.5 ug/ml)
Target Species
Human
Immunogen Description
Synthetic 15 amino acid peptide from internal region of human GCNT3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Panda, Horse (100%); Marmoset, Sheep, Bovine, Pig (93%); Hamster, Rabbit, Platypus.
Immunogen Type
Synthetic peptide
Immunogen
GCNT3 antibody was raised against synthetic 15 amino acid peptide from internal region of human GCNT3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Panda, Horse (100%); Marmoset, Sheep, Bovine, Pig (93%); Hamster, Rabbit, Platypus, Xenopus (80%).
Antigen Modification
Internal
Preparation and Storage
Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-GCNT3 antibody IHC of human stomach. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-GCNT3 antibody IHC of human stomach. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-GCNT3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-GCNT3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Related Product Information for anti-GCNT3 antibody
GCNT3 is a Beta-1, 6-N-Acetylglucosaminyltranscerase that catalyzes O-glycan branch formation of the core 2 and core 4 type. The expression of core 2 O-glycans has been correlated with cell differentiation processes and cancer.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,864 Da
NCBI Official Full Name
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
NCBI Official Synonym Full Names
glucosaminyl (N-acetyl) transferase 3, mucin type
NCBI Official Symbol
GCNT3
NCBI Official Synonym Symbols
GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
NCBI Protein Information
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; hC2GnT-M; C2GnT-mucin type; beta1,6GlcNAc-transferase; beta1,6-N-acetylglucosaminyltransferase-M; core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
UniProt Protein Name
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
UniProt Gene Name
GCNT3
UniProt Synonym Gene Names
C2GnT-M; hC2GnT-M; C2/4GnT
UniProt Entry Name
GCNT3_HUMAN

NCBI Description

This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]

Uniprot Description

GCNT3: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. Belongs to the glycosyltransferase 14 family.

Protein type: EC 2.4.1.150; Glycan Metabolism - O-glycan biosynthesis; Transferase; Membrane protein, integral; EC 2.4.1.102

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity; acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; tissue morphogenesis; cellular protein metabolic process; O-glycan processing; immunoglobulin production in mucosal tissue; carbohydrate metabolic process; post-translational protein modification; intestinal absorption

Research Articles on GCNT3

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Product Notes

The GCNT3 gcnt3 (Catalog #AAA243316) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human GCNT3 reacts with Gibbon, Dog, Gorilla, Horse, Human Predicted Reactivity: Monkey, Bovine, Pig, Sheep (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's GCNT3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin). IHC-P (5 - 7.5 ug/ml). Researchers should empirically determine the suitability of the GCNT3 gcnt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCNT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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