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Western Blot (WB) (Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CACT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Rat Carnitine Acylcarnitine Translocase Polyclonal Antibody | anti-CACT antibody

Carnitine Acylcarnitine Translocase (CACT) Polyclonal Antibody

Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Carnitine Acylcarnitine Translocase; Polyclonal Antibody; Carnitine Acylcarnitine Translocase (CACT) Polyclonal Antibody; SLC25A20; CAC; Solute Carrier Family 25 Member 20; Carnitine Acylcarnitine Carrier; Mitochondrial carnitine/acylcarnitine carrier protein; anti-CACT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CACT. It has been selected for its ability to recognize CACT in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-CACT antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2ug/mL;1:500-2000
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Rat
Immunogen
Recombinant CACT (Met1~Arg211) expressed in E.coli
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2057649
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CACT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CACT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Liver Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Liver Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
33,154 Da
NCBI Official Full Name
Mitochondrial carnitine/acylcarnitine carrier protein
NCBI Official Synonym Full Names
solute carrier family 25 member 20
NCBI Official Symbol
Slc25a20
NCBI Protein Information
mitochondrial carnitine/acylcarnitine carrier protein
UniProt Protein Name
Mitochondrial carnitine/acylcarnitine carrier protein
Protein Family
UniProt Gene Name
Slc25a20
UniProt Synonym Gene Names
Cact; CAC

NCBI Description

carnitine carrier protein, component of the mitochondrial inner membrane; trasfers fatty acylcarnitines into mitochondria; critical for fatty acid oxidation [RGD, Feb 2006]

Uniprot Description

SLC25A20: Mediates the transport of acylcarnitines of different length across the mitochondrial inner membrane from the cytosol to the mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 8q32

Cellular Component: cytosol; integral component of membrane; mitochondrial inner membrane; mitochondrion

Molecular Function: acyl carnitine transmembrane transporter activity; carnitine transmembrane transporter activity; carnitine:acyl carnitine antiporter activity

Biological Process: carnitine transport; mitochondrial transport; transport

Research Articles on CACT

Similar Products

Product Notes

The CACT slc25a20 (Catalog #AAA2032205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Carnitine Acylcarnitine Translocase (CACT) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Carnitine Acylcarnitine Translocase can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2ug/mL;1:500-2000 Immunohistochemistry: 5-20ug/mL;1:50-200 Immunocytochemistry: 5-20ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CACT slc25a20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carnitine Acylcarnitine Translocase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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