Ubiquitin Specific Peptidase 8 Recombinant Protein | USP8 recombinant protein

Recombinant Ubiquitin Specific Peptidase 8 (USP8)

Cat. Num. AAA2031664

• Gene Names: USP8; UBPY; SPG59; HumORF8
• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Ubiquitin Specific Peptidase 8; Recombinant Ubiquitin Specific Peptidase 8 (USP8); USP8 recombinant protein

• Host: E.coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: > 90%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 300ug/mL (varies by lot)
• Sequence: TGLR NLGNTCYMNS ILQCLCNAPH LADYFNRNCY QDDINRSNLL GHKGEVAEEF GIIMKALWTG QYRYISPKDF KITIGKINDQ FAGYSQQDSQ ELLLFLMDGL HEDLNKADNR KRYKEENNDH LDDFKAAEHA WQKHKQLNES IIVALFQGQF KSTVQCLTCH KKSRTFEAFM YLSLPLASTS KCTLQDCLRL FSKEEKLTDN NRFYCSHCRA RRDSLKKIEI WKLPPVLLVH LKRFSYDGRW KQKLQTSVDF PLENLDLSQY VIGPKNNLKK YNLFSVSNHY GGLDGGHYTA YCKNAARQRW FKFDDHEVSD ISVSSVKSSA AYILFYTSLG PRVTDVAT

• Applicable Applications for USP8 recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Thr777~Thr1118
• Tags: N-terminal His Tag
• Subcellular Location: Membrane, Nucleus, Cytoplasm
• Traits: Freeze-dried powder
• Predicted isoelectric point: 8.8
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 83405557
• NCBI GeneID: 9101
• Molecular Weight: Predicted Molecular Mass: 43.3kDa; Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: Ubiquitin specific peptidase 8
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 8
• NCBI Official Symbol: USP8
• NCBI Official Synonym Symbols: UBPY; SPG59; HumORF8
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 8
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 8
• UniProt Gene Name: USP8
• UniProt Synonym Gene Names: KIAA0055; UBPY; hUBPy
• UniProt Entry Name: UBP8_HUMAN

NCBI Description

This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

USP8: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1. Upon growth stimulation in starved human fibroblasts. Decreases in response to growth arrest induced by cell-cell contact. Belongs to the peptidase C19 family.

Protein type: Ubiquitin-specific protease; Ubiquitin conjugating system; EC 3.4.19.12; Protease

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: extrinsic to plasma membrane; nucleoplasm; Golgi apparatus; cytoplasm; early endosome; acrosome; midbody; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SH3 domain binding

Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; endosome organization and biogenesis; cytokinesis after mitosis; Ras protein signal transduction