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Inter Alpha-Globulin Inhibitor H3 Recombinant Protein | ITIH3 recombinant protein

Recombinant Inter Alpha-Globulin Inhibitor H3 (ITIH3)

Gene Names
ITIH3; H3P; SHAP; ITI-HC3
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Inter Alpha-Globulin Inhibitor H3; Recombinant Inter Alpha-Globulin Inhibitor H3 (ITIH3); ITIH3 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
Original Concentration : 200 Ug/mL (varies by lot)
Sequence Length
890
Applicable Applications for ITIH3 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.

(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Pro279~Val467
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Buffer formulation
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Predicted isoelectric point
5.5
Predicted Molecular Mass
25.2kDa
Accurate Molecular Mass
26kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H3 preproprotein
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 3
NCBI Official Symbol
ITIH3
NCBI Official Synonym Symbols
H3P; SHAP; ITI-HC3
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H3
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H3
UniProt Gene Name
ITIH3
UniProt Synonym Gene Names
ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; SHAP
UniProt Entry Name
ITIH3_HUMAN

NCBI Description

This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3. [provided by RefSeq, Jul 2015]

Uniprot Description

ITIH3: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: hyaluronan metabolic process

Research Articles on ITIH3

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Product Notes

The ITIH3 itih3 (Catalog #AAA2031080) is a Recombinant Protein produced from Host: E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Inter Alpha-Globulin Inhibitor H3 (ITIH3) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Inter Alpha-Globulin Inhibitor H3 can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, WB.

(May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the ITIH3 itih3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Inter Alpha-Globulin Inhibitor H3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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