AGR 2 recombinant protein

AGR 2, 21-175aa, Human, His tag, E Coli

Cat. Num. AAA203107

• Gene Names: AGR2; AG2; AG-2; HPC8; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: AGR 2; 21-175aa; Human; His tag; E Coli; Anterior gradient 2 homolog; AG2; GOB-4; HAG-2; XAG-2; AGR2; anterior gradient homolog 2 (Xenopus laevis); PDIA17; protein disulfide isomerase family A member 17; Anterior Gradient Protein 2 Homolog; AGR 2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: 20mM Tris 8.0, 1mM DTT, 10% glycerol, 1mM EDTA
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSRDTT VKPGAKKDTK DSRPKLPQTL SRGWGDQLIW TQTYEEALYK SKTSNKPLMI IHHLDECPHS QALKKVFAEN KEIQKLAEQF VLLNLVYETT DKHLSPDGQY VPRIMFVDPS LTVRADITGR YSNRLYAYEP ADTALLLDNM KKALKLLKTE L
• Sequence Length: 175

• Applicable Applications for AGR 2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for AGR 2 recombinant protein

AGR2 (Anterior gradient 2 homolog) is the human orthologue of the secreted Xenopus laevis Anterior Gradient protein (XAG-2). This is a small, possibly secreted molecule of yet weakly defined functions that is widely expressed in human tissues. Expression of AGR2 shows a positive correlation with expression of estrogen receptor in breast carcinoma and a negative correlation with expression of EGF receptor. Recombinant human AGR2, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for AGR 2 recombinant protein

Cancer

References

F.R. Fritzsche. et al., (2007). Histol Histopathol. 22: 703-708; Fletcher GC. et al., (2003) Br J Cancer 88 (4): 579-85 ; Liu D. et al., (2005) Cancer Res. 65 : 3796-805;

NCBI and Uniprot Product Information

• NCBI GI #: 5453541
• NCBI GeneID: 10551
• NCBI Accession #: NP_006399
• NCBI GenBank Nucleotide #: NM_006408.3
• UniProt Accession #: O95994
• Molecular Weight: 21.9 kDa (191 aa), confirmed by MALDI-TOF
• NCBI Official Full Name: anterior gradient protein 2 homolog
• NCBI Official Synonym Full Names: anterior gradient 2, protein disulphide isomerase family member
• NCBI Official Symbol: AGR2
• NCBI Official Synonym Symbols: AG2; AG-2; HPC8; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
• NCBI Protein Information: anterior gradient protein 2 homolog
• UniProt Protein Name: Anterior gradient protein 2 homolog
• UniProt Gene Name: AGR2
• UniProt Synonym Gene Names: AG2; AG-2; hAG-2

NCBI Description

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and a C-terminal ER-retention sequence. This protein plays a role in cell migration, cellular transformation and metastasis and is as a p53 inhibitor. As an ER-localized molecular chaperone, it plays a role in the folding, trafficking, and assembly of cysteine-rich transmembrane receptors and the cysteine-rich intestinal gylcoprotein mucin. This gene has been implicated in inflammatory bowel disease and cancer progression. [provided by RefSeq, Mar 2017]

Uniprot Description

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed:23274113).