Western Blot (WB)
(Western Blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.)
Rabbit anti-Human AGR2 Polyclonal Antibody | anti-AGR2 antibody
AGR2 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
AGR2; Polyclonal Antibody; AGR2 Polyclonal Antibody; AG-2; hAG-2; AG 2; AG 2 protein; AG2; AG2 protein; AGR 2; Anterior gradient 2 homolog (Xenopus laevis); anterior gradient 2 homolog; Anterior gradient 2; Xenopus; homolog of; Anterior gradient homolog 2; Anterior gradient protein 2 homolog; GOB 4; GOB4; HAG 2; hAG2; HAG2/R; HPC 8; hPC8; PDIA17; Protein disulfide isomerase family A member 17; secreted cement gland homolog; Secreted cement gland protein XAG 2 homolog; Secreted cement gland protein XAG-2 homolog; Secreted cement gland protein XAG2 homolog; XAG 2; XAG2; anti-AGR2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
AGR2 polyclonal antibody detects endogenous levels of AGR2 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
175
Applicable Applications for anti-AGR2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000-1:5,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:100-1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-AGR2 antibody
AGR2 (Anterior Gradient Protein 2), also known as AG2, GOB-4 or HAG-2, is a member of the anterior gradient homolog family. It is the human ortholog of XAG-2, the secreted Xenopus laevis Anterior Gradient protein. In X. laevis, it is involved in cement gland differentiation and neural marker gene expression. AGR2 is a secretory protein encoded by two different AGR2 transcripts. It interacts with LYPD3 and alpha-dystroglycan (DAG-1). AGR2 is ubiquitously expressed with up-regulated expression in prostate cancer, breast cancer, lung cancer, renal carcinomas and endometrial carcinomas. AGR2 expression is positively correlated with that of the estrogen receptor (ER) and is negatively correlated with that of the epidermal growth factor receptor (EGFR). AGR2 may serve as a potential therapeutic marker for various cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17kDa
NCBI Official Full Name
anterior gradient protein 2 homolog
UniProt Protein Name
Anterior gradient protein 2 homolog
Protein Family
UniProt Gene Name
AGR2
UniProt Synonym Gene Names
AG2; AG-2; hAG-2
UniProt Entry Name
AGR2_HUMAN
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Product Notes
The AGR2 agr2 (Catalog #AAA3012207) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGR2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AGR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1,000-1:5,000 ICC: 1:100-1:500 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the AGR2 agr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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