Interleukin 7 Recombinant Protein | IL7 recombinant protein

Recombinant Interleukin 7 (IL7)

Cat. Num. AAA2030647

• Gene Names: IL7; IL-7
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Interleukin 7; Recombinant Interleukin 7 (IL7); IL7 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; NCDIE KIKHGKEYEN VLMISINELD KMIEIGSNCL NNESNFFKKH SCDDNKEADF LERAARKLDQ FHDMSINEDF QNHLERVSKG TLTLVNCTGK EEKKPLKDQK KNGRCFLKKLLQKIKTCWNK ILRGIKEN
• Sequence Length: 177

• Applicable Applications for IL7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 19.4KDa
• Accurate Molecular Mass (KD): 19kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Met21~Lys171
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4504677
• NCBI GeneID: 3574
• NCBI Accession #: NP_000871.1
• NCBI GenBank Nucleotide #: NM_000880.3
• Molecular Weight: 7,687 Da
• NCBI Official Full Name: interleukin-7 isoform 1
• NCBI Official Synonym Full Names: interleukin 7
• NCBI Official Symbol: IL7
• NCBI Official Synonym Symbols: IL-7
• NCBI Protein Information: interleukin-7
• UniProt Protein Name: Interleukin-7
• Protein Family: Interleukin
• UniProt Gene Name: IL7
• UniProt Synonym Gene Names: IL-7
• UniProt Entry Name: IL7_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

Uniprot Description

IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide; Cytokine; Apoptosis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q12-q13

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: positive regulation of organ growth; humoral immune response; T cell lineage commitment; organ morphogenesis; cell-cell signaling; positive regulation of T cell differentiation; regulation of gene expression; homeostasis of number of cells within a tissue; negative regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of B cell proliferation; negative regulation of apoptosis; bone resorption