Western Blot (WB)
(Western Blot analysis of PIP4K2A expression in transfected 293T cell line by PIP4K2A monoclonal antibody (M08), clone 3H5.Lane 1: PIP4K2A transfected lysate (46.2 KDa).Lane 2: Non-transfected lysate.)
Mouse PIP4K2A Monoclonal Antibody | anti-PIP4K2A antibody
PIP4K2A (Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, alpha, FLJ13267, PI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK) (PE)
Gene Names
PIP4K2A; PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified
Synonyms
PIP4K2A; Monoclonal Antibody; PIP4K2A (Phosphatidylinositol-5-Phosphate 4-Kinase; Type II; alpha; FLJ13267; PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK) (PE); Phosphatidylinositol-5-Phosphate 4-Kinase; PIPK; anti-PIP4K2A antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3H5
Specificity
Recognizes PIP4K2A.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
406
Applicable Applications for anti-PIP4K2A antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
PIP4K2A (AAH18034, 1aa-406aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of PIP4K2A expression in transfected 293T cell line by PIP4K2A monoclonal antibody (M08), clone 3H5.Lane 1: PIP4K2A transfected lysate (46.2 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PIP4K2A expression in transfected 293T cell line by PIP4K2A monoclonal antibody (M08), clone 3H5.Lane 1: PIP4K2A transfected lysate (46.2 KDa).Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to PIP4K2A on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to PIP4K2A on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to PIP4K2A on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to PIP4K2A on HeLa cell. [antibody concentration 10 ug/ml])
Related Product Information for anti-PIP4K2A antibody
Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq]
Product Categories/Family for anti-PIP4K2A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
NCBI Official Synonym Full Names
phosphatidylinositol-5-phosphate 4-kinase type 2 alpha
NCBI Official Symbol
PIP4K2A
NCBI Official Synonym Symbols
PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
NCBI Protein Information
phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
Protein Family
NCBI Description
Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]
Research Articles on PIP4K2A
Similar Products
Product Notes
The PIP4K2A (Catalog #AAA6186911) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PIP4K2A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PIP4K2A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIP4K2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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