Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 Recombinant Protein | AIMP1 recombinant protein

Recombinant Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1)

Cat. Num. AAA2029636

• Gene Names: AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1; Recombinant Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1); AIMP1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 8.7 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATNDAVLKR LEQKGAEADQ IIEYLKQQVA LLKEKAILQA TMREEKKLRV ENAKLKKEIE ELKQELILAE IHNGVEQVRV RLSTPLQTNC TASESVVQSP SVATTASPAT KEQIKAGEEK KVKEKTEKKG EKKEKQQSAA ASTDSKPIDA SRLDLRIGCI VTAKKHPDAD SLYVEEVDVG EAAPRTVVSG LVNHVPLEQM QNRMVVLLCN LKPAKMRGVL SQAMVMCASS PEKVEILAPP NGSVPGDRIT FDAFPGEPDKELNPKKKIWE QIQPDLHTNA ECVATYKGAP FEVKGKGVCR AQTMANSGIK
• Sequence Length: 312

• Applicable Applications for AIMP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 37.7kDa
• Accurate Molecular Mass (KD): 42kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Pro27~Ser158
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 45006986
• NCBI GeneID: 9255
• NCBI Accession #: NP_004748.2
• NCBI GenBank Nucleotide #: NM_004757.3
• Molecular Weight: 37,039 Da
• NCBI Official Full Name: aminoacyl tRNA synthase complex-interacting multifunctional protein 1 isoform a
• NCBI Official Synonym Full Names: aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
• NCBI Official Symbol: AIMP1
• NCBI Official Synonym Symbols: p43; HLD3; EMAP2; SCYE1; EMAPII
• NCBI Protein Information: aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• UniProt Protein Name: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• Protein Family: Aminoacyl tRNA synthase complex-interacting multifunctional protein
• UniProt Gene Name: AIMP1
• UniProt Synonym Gene Names: EMAP2; SCYE1; EMAP-2; EMAP-II
• UniProt Entry Name: AIMP1_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]

Uniprot Description

SCYE1 iso2: Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7. Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: Golgi apparatus; extracellular space; aminoacyl-tRNA synthetase multienzyme complex; transport vesicle; cell surface; membrane; endoplasmic reticulum; cytoplasm; cytosol; nucleus

Molecular Function: methionine-tRNA ligase activity; protein binding; protein homodimerization activity; cytokine activity; tRNA binding

Biological Process: apoptosis; methionyl-tRNA aminoacylation; glucose metabolic process; signal transduction; chemotaxis; tRNA aminoacylation for protein translation; cell-cell signaling; negative regulation of endothelial cell proliferation; response to wounding; gene expression; angiogenesis; inflammatory response; cell adhesion; leukocyte migration

Disease: Leukodystrophy, Hypomyelinating, 3