Programmed Cell Death Protein 6 Recombinant Protein | PDCD6IP recombinant protein

Recombinant Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP)

Cat. Num. AAA2029457

• Gene Names: PDCD6; ALG2; ALG-2; PEF1B
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Programmed Cell Death Protein 6; Recombinant Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP); PDCD6IP recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; EPTVDIS PDTVGTLSLI MLAQAQEVFF LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKY FYFQEVFPVL AAKHCIMQAN AEYHQSILAK QQKKFGEEIA RLQHAAELIK TVASRYDEYV NVKDFSDKIN RALAAAKKDN DFIYHDRVPD LKDLDPIGKA TLVKSTPVNVPISQKFTDLF EKMVPVSVQQ SLAAYNQRKA DLV
• Sequence Length: 69

• Applicable Applications for PDCD6IP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 53.8kDa
• Accurate Molecular Mass (KD): 54kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, GST-tag, S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Glu174~Val383
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 389565489
• NCBI GeneID: 10016
• NCBI Accession #: NP_001254488.1
• NCBI GenBank Nucleotide #: NM_001267559.1
• Molecular Weight: 7,349 Da
• NCBI Official Full Name: programmed cell death protein 6 isoform 5
• NCBI Official Synonym Full Names: programmed cell death 6
• NCBI Official Symbol: PDCD6
• NCBI Official Synonym Symbols: ALG2; ALG-2; PEF1B
• NCBI Protein Information: programmed cell death protein 6
• UniProt Protein Name: Programmed cell death protein 6
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD6
• UniProt Synonym Gene Names: ALG2
• UniProt Entry Name: PDCD6_HUMAN

NCBI Description

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5. [provided by RefSeq, May 2012]

Uniprot Description

PDCD6: Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)- regulated signals along the death pathway. Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity.

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; cytoplasm; cytoplasmic vesicle; nucleus; endosome

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; protein anchor; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; calcium-dependent protein binding; molecular adaptor activity

Biological Process: caspase activation; intracellular protein transport; negative regulation of TOR signaling pathway; positive regulation of angiogenesis; negative regulation of protein kinase B signaling cascade; negative regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of endothelial cell proliferation; angiogenesis; proteolysis; response to calcium ion