Tau-Tubulin Kinase 2 Recombinant Protein | tTBK2 recombinant protein

Recombinant Tau-Tubulin Kinase 2 (tTBK2)

Cat. Num. AAA2029449

• Gene Names: TTBK2; TTBK; SCA11
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Tau-Tubulin Kinase 2; Recombinant Tau-Tubulin Kinase 2 (tTBK2); tTBK2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; WKVLRKIGGG GFGEIYDALD MLTRENVALKVESAQQPKQV LKMEVAVLKK LQGKDHVCRF IGCGRNDRFN YVVMQLQGRNLADLRRSQSR GTFTISTTLR LGRQILESIE SIHSVGFLHR DIKPSNFAMGRFPSTCRKCY MLDFGLARQF TNSCGDVRPP RAVAGFRGTV RYASINAHRNREMGRHDDLW SLFYMLVEFV VGQLPWRKIK DKEQVGSIKE RYDHRLMLKHLPPEFSIFLD HISSLDYFTK PDYQLLTSVF DNSIKTFGVI ESDPFDWEKTGNDGSLTTTT TSTT
• Sequence Length: 1244

• Applicable Applications for tTBK2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 63.7kDa
• Accurate Molecular Mass (KD): 64kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Trp21~Thr314
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 116812624
• NCBI GeneID: 146057
• NCBI Accession #: NP_775771.3
• NCBI GenBank Nucleotide #: NM_173500.3
• Molecular Weight: 54,037 Da
• NCBI Official Full Name: tau-tubulin kinase 2
• NCBI Official Synonym Full Names: tau tubulin kinase 2
• NCBI Official Symbol: TTBK2
• NCBI Official Synonym Symbols: TTBK; SCA11
• NCBI Protein Information: tau-tubulin kinase 2
• UniProt Protein Name: Tau-tubulin kinase 2
• Protein Family: Tau-tubulin kinase
• UniProt Gene Name: TTBK2
• UniProt Synonym Gene Names: KIAA0847
• UniProt Entry Name: TTBK2_HUMAN

NCBI Description

This gene encodes a serine-threonine kinase that putatively phosphorylates tau and tubulin proteins. Mutations in this gene cause spinocerebellar ataxia type 11 (SCA11); a neurodegenerative disease characterized by progressive ataxia and atrophy of the cerebellum and brainstem. [provided by RefSeq, Aug 2009]

Uniprot Description

TTBK2: Serine/threonine kinase which is able to phosphorylate tau on serines. Defects in TTBK2 are the cause of spinocerebellar ataxia type 11 (SCA11). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA11 is an autosomal dominant cerebellar ataxia (ADCA). It is a relatively benign, late-onset, slowly progressive neurologic disorder. Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CK1; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CK1 group; TTBK family

Chromosomal Location of Human Ortholog: 15q15.2

Cellular Component: centriole; extracellular space; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: regulation of cell shape; smoothened signaling pathway; peptidyl-serine phosphorylation; organelle organization and biogenesis; cilium biogenesis

Disease: Spinocerebellar Ataxia 11