Gene Sequencing (Extract)
Secretogranin II Recombinant Protein | SCG2 recombinant protein
Recombinant Secretogranin II (SCG2)
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Secretogranin II; Recombinant Secretogranin II (SCG2); SCG2 recombinant protein
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 90%
Form/Format
20mM Tris, 500mM NaCl, pH8.0, containing 5% Trehalose and Proclin300.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
QRNQLLQKEP DLRLENVQKF PSPEMIRALE YIENLRQQAH KEESSPDYNP YQGVSVPLQQ KENGDESHLP ERDSLSEEDW MRIILEALRQ AENEPQSAPK ENKPYALNSE KNFPMDMSDD YETQQWPERK LKHMQFPPMY EENSRDNPFK RTNEIVEEQY TPQSLATLES VFQELGKLTG PNNQKRERMD EEQKLYTDDE DDIYKANNIA YEDVVGGEDW NPVEEKIESQ TQEEVRDSKE NIEKNEQIND EMKRSGQLGI QEEDLRKESK DQLSDDVSKV IAYLKRLVNA AGSGRLQNGQ NGERATRLFE KPLDSQSIYQ LIEISRNLQI PPEDLIEMLK TGEKPNGSVE PERELDLPVD LDDISEADLD HPDLFQNRML SKSGYPKTPG RAGTEALPDG LSVEDILNLL GMESAANQKT SYFPNPYNQE KVLPRLPYGA GRSRSNQLPK AAWIPHVENR QMAYENLNDK DQELGEYLAR MLVKYPEIIN SNQVKRVPGQ GSSEDDLQEE EQIEQAIKEH LNQGSSQETD KLAPVSKRFP VGPPKNDDTP NRQYWDEDLL MKVLEYLNQE KAEKGREHIA KRAMENM
Applicable Applications for SCG2 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Gln31~Met617
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
4.7
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Gene Sequencing (Extract)
Gene Sequencing (Extract)
SDS-PAGE
SDS-PAGE
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
Predicted Molecular Mass: 71.5 kDa
Accurate Molecular Mass: 72kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 72kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
secretogranin II
UniProt Protein Name
Secretogranin-2
Protein Family
UniProt Gene Name
SCG2
UniProt Synonym Gene Names
CHGC; SgII; SN
UniProt Entry Name
SCG2_HUMAN
Uniprot Description
SCG2: Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. Belongs to the chromogranin/secretogranin protein family.
Protein type: Cell cycle regulation; Apoptosis; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2q35-q36
Cellular Component: extracellular space; secretory granule
Molecular Function: chemoattractant activity; cytokine activity; protein binding
Biological Process: angiogenesis; endothelial cell migration; eosinophil chemotaxis; induction of positive chemotaxis; inflammatory response; MAPKKK cascade; negative regulation of endothelial cell proliferation; positive chemotaxis; positive regulation of endothelial cell proliferation; protein secretion
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Product Notes
The SCG2 scg2 (Catalog #AAA2028995) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Secretogranin II (SCG2) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Secretogranin II can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the SCG2 scg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRNQLLQKEP DLRLENVQKF PSPEMIRALE YIENLRQQAH KEESSPDYNP YQGVSVPLQQ KENGDESHLP ERDSLSEEDW MRIILEALRQ AENEPQSAPK ENKPYALNSE KNFPMDMSDD YETQQWPERK LKHMQFPPMY EENSRDNPFK RTNEIVEEQY TPQSLATLES VFQELGKLTG PNNQKRERMD EEQKLYTDDE DDIYKANNIA YEDVVGGEDW NPVEEKIESQ TQEEVRDSKE NIEKNEQIND EMKRSGQLGI QEEDLRKESK DQLSDDVSKV IAYLKRLVNA AGSGRLQNGQ NGERATRLFE KPLDSQSIYQ LIEISRNLQI PPEDLIEMLK TGEKPNGSVE PERELDLPVD LDDISEADLD HPDLFQNRML SKSGYPKTPG RAGTEALPDG LSVEDILNLL GMESAANQKT SYFPNPYNQE KVLPRLPYGA GRSRSNQLPK AAWIPHVENR QMAYENLNDK DQELGEYLAR MLVKYPEIIN SNQVKRVPGQ GSSEDDLQEE EQIEQAIKEH LNQGSSQETD KLAPVSKRFP VGPPKNDDTP NRQYWDEDLL MKVLEYLNQE KAEKGREHIA KRAMENM. It is sometimes possible for the material contained within the vial of "Secretogranin II, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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