Western Blot (WB)
(Western Blot: Sample: Human Serum; Primary Ab: 3ug/ml Mouse Anti-Porcine APOA4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Mouse anti-Pig Apolipoprotein A4 Monoclonal Antibody | anti-APOA4 antibody
Monoclonal Antibody to Apolipoprotein A4 (APOA4)
Reactivity
Pig
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Apolipoprotein A4; Monoclonal Antibody; Monoclonal Antibody to Apolipoprotein A4 (APOA4); anti-APOA4 antibody
Host
Mouse
Reactivity
Pig
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
A8
Specificity
The antibody is a mouse monoclonal antibody raised against APOA4. It has beenselected for its ability to recognize APOA4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
1mg/mL (varies by lot)
Sequence Length
395
Applicable Applications for anti-APOA4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:500-5000
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Organism Species
Sus scrofa; Porcine (Pig)
Cross Reactivity
Pig
Conjugate
No Conjugate
Immunogen
Recombinant APOA4 (Glu21~Gly382) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2061232
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human Serum; Primary Ab: 3ug/ml Mouse Anti-Porcine APOA4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Serum; Primary Ab: 3ug/ml Mouse Anti-Porcine APOA4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,294 Da
NCBI Official Full Name
apolipoprotein A-IV
NCBI Official Symbol
APOA4
NCBI Protein Information
apolipoprotein A-IV
UniProt Protein Name
Apolipoprotein A-IV
Protein Family
UniProt Gene Name
APOA4
UniProt Synonym Gene Names
Apo-AIV; ApoA-IV
Uniprot Description
May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Research Articles on APOA4
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Product Notes
The APOA4 apoa4 (Catalog #AAA2025725) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Apolipoprotein A4 (APOA4) reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein A4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:500-5000 Immunohistochemistry: 5-20ug/mL;1:50-200 Immunocytochemistry: 5-20ug/mL;1:50-200 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the APOA4 apoa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein A4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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