Typical Testing Data/Standard Curve (for reference only)
Human Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit | TNFSF4 elisa kit
Human Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TNFSF4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TNFSF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFSF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFSF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
TNFSF4: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T-cell-APC interaction and the functional consequences of T-cell activation, thereby destabilizing peripheral tolerance. Belongs to the tumor necrosis factor family.
Protein type: Cytokine; Membrane protein, integral; Cell cycle regulation
Chromosomal Location of Human Ortholog: 1q25
Cellular Component: extracellular space; cell surface; integral to plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding
Biological Process: negative regulation of T-helper 1 cell differentiation; positive regulation of interleukin-12 production; negative regulation of cytokine secretion; positive regulation of T cell cytokine production; defense response to nematode; positive regulation of activated T cell proliferation; signal transduction; positive regulation of interleukin-10 production; positive regulation of interleukin-4 production; T cell proliferation; regulation of adaptive immune response; positive regulation of T-helper 2 cell differentiation; negative regulation of regulatory T cell differentiation; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; positive regulation of memory T cell differentiation; cholesterol metabolic process; positive regulation of T-helper 2 type immune response; response to virus; negative regulation of transcription factor activity; positive regulation of immunoglobulin mediated immune response; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; positive regulation of CD4-positive, alpha beta T cell differentiation; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of interleukin-2 production; immune response; positive regulation of alpha-beta T cell proliferation; negative regulation of transcription, DNA-dependent; acute inflammatory response; positive regulation of inflammatory response
Disease: Myocardial Infarction, Susceptibility To
Research Articles on TNFSF4
Similar Products
Product Notes
The Human TNFSF4 tnfsf4 (Catalog #AAA2023072) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023072 ELISA Kit recognizes Human TNFSF4. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
