Human Annexin A11 (ANXA11) ELISA Kit | ANXA11 elisa kit

Human Annexin A11 (ANXA11) ELISA Kit

Cat. Num. AAA2022052

• Reactivity: Human
• Synonyms: Annexin A11 (ANXA11); Human Annexin A11 (ANXA11) ELISA Kit; ANX-A11; ANX11; CAP50; 56 kDa autoantigen; Calcyclin-associated annexin 50; ANXA11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANP. No significant cross-reactivity or interference between ANP and analogues was observed.
• Sequence Length: 472

• Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8-500pg/mL
• Sensitivity: 3.0pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANXA11 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANP in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ANP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ANP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ANXA11 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 8671175
• UniProt Accession #: P50995; B4DVE7
• Molecular Weight: 51,242 Da
• NCBI Official Full Name: annexin A11
• UniProt Protein Name: Annexin A11
• Protein Family: Annexin
• UniProt Gene Name: ANXA11
• UniProt Synonym Gene Names: ANX11; CAP-50
• UniProt Entry Name: ANX11_HUMAN

Uniprot Description

ANXA11: a calcium/phospholipid-binding protein found throughout the nucleoplasm at interphase and at the mitotic phase is concentrated at the loop-like structure around the mitotic apparatus. May interact with calcyclin (S100A6) in vivo. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Motility/polarity/chemotaxis; Calcium-binding; Nuclear envelope

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleoplasm; azurophil granule; specific granule; membrane; cytoplasm; melanosome; spindle; nuclear envelope; midbody; phagocytic vesicle

Molecular Function: protein binding; calcium-dependent phospholipid binding; phosphatidylethanolamine binding; calcium ion binding; calcium-dependent protein binding

Biological Process: response to calcium ion; phagocytosis