Typical Testing Data/Standard Curve (for reference only)
Human Phospholipase A2, Group XIIB (PLA2G12B) ELISA Kit | PLA2G12B elisa kit
Human Phospholipase A2, Group XIIB (PLA2G12B) ELISA Kit
Gene Names
PLA2G12B; GXIIB; FKSG71; PLA2G13; GXIIIsPLA2
Reactivity
Human
Synonyms
Phospholipase A2; Group XIIB (PLA2G12B); Human Phospholipase A2; Group XIIB (PLA2G12B) ELISA Kit; PLA2G13; FKSG71; GXIIB; Group XIII; Group XIIB secretory phospholipase A2-like protein; PLA2G12B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group XIIB (PLA2G12B).
No significant cross-reactivity or interference between Phospholipase A2, Group XIIB (PLA2G12B) and analogues was observed.
No significant cross-reactivity or interference between Phospholipase A2, Group XIIB (PLA2G12B) and analogues was observed.
Sequence Length
195
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Phospholipase A2, Group XIIB (PLA2G12B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Phospholipase A2, Group XIIB (PLA2G12B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLA2G12B elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group XIIB (PLA2G12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group XIIB (PLA2G12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for PLA2G12B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,588 Da
NCBI Official Full Name
Phospholipase A2, group XIIB
NCBI Official Synonym Full Names
phospholipase A2, group XIIB
NCBI Official Symbol
PLA2G12B
NCBI Official Synonym Symbols
GXIIB; FKSG71; PLA2G13; GXIIIsPLA2
NCBI Protein Information
group XIIB secretory phospholipase A2-like protein
UniProt Protein Name
Group XIIB secretory phospholipase A2-like protein
Protein Family
UniProt Gene Name
PLA2G12B
UniProt Synonym Gene Names
PLA2G13; GXIII sPLA2-like; GXIIB
UniProt Entry Name
PG12B_HUMAN
NCBI Description
Phospholipase A2 (PLA2) enzymes catalyze hydrolysis of glycolipids to release free fatty acids and lysophospholipids. PLA2G12B belongs to the PLA2 family, but it is catalytically inactive due to an amino acid change in its active site and has altered phospholipid-binding properties (Rouault et al., 2003 [PubMed 14516201]).[supplied by OMIM, Mar 2008]
Uniprot Description
PLA2G12B: Not known; does not seem to have catalytic activity. Belongs to the phospholipase A2 family.
Protein type: Secreted; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; Secreted, signal peptide; Lipid Metabolism - ether lipid; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 10q22.1
Cellular Component: extracellular region
Molecular Function: phospholipase A2 activity; calcium ion binding
Biological Process: cholesterol homeostasis; lipid catabolic process
Research Articles on PLA2G12B
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Product Notes
The Human PLA2G12B pla2g12b (Catalog #AAA2020295) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2020295 ELISA Kit recognizes Human PLA2G12B. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Group XIIB (PLA2G12B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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