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Typical Testing Data/Standard Curve (for reference only)

Rat Reelin (RL) ELISA Kit | RL elisa kit

Rat Reelin (RL) ELISA Kit

Gene Names
RELN; RL; ETL7; LIS2; PRO1598
Reactivity
Rat
Synonyms
Reelin (RL); Rat Reelin (RL) ELISA Kit; RELN; RL elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Reelin (RL).
No significant cross-reactivity or interference between Reelin (RL) and analogues was observed.
Sequence Length
3460
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Reelin (RL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Reelin (RL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RL elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reelin (RL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Reelin (RL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Reelin (RL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reelin (RL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for RL elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
384,093 Da
NCBI Official Full Name
reelin
NCBI Official Synonym Full Names
reelin
NCBI Official Symbol
RELN
NCBI Official Synonym Symbols
RL; ETL7; LIS2; PRO1598
NCBI Protein Information
reelin
UniProt Protein Name
Reelin
UniProt Gene Name
RELN
UniProt Entry Name
RELN_HUMAN

NCBI Description

This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

reelin: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2); also known as lissencephaly with cerebellar hypoplasia or Norman-Roberts syndrome. LIS2 is a classic type lissencephaly associated with abnormalities of the cerebellum, hippocampus and brainstem. Individuals with LIS2 are severely ataxic, mentally retarded and suffer from epilepsy. Belongs to the reelin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; EC 3.4.21.-; Protease; Cell development/differentiation; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: proteinaceous extracellular matrix; extracellular space; cytoplasm; dendrite

Molecular Function: serine-type peptidase activity; metal ion binding; protein serine/threonine/tyrosine kinase activity

Biological Process: regulation of synaptic transmission; axon guidance; cellular morphogenesis during differentiation; peptidyl-tyrosine phosphorylation; central nervous system development; cerebral cortex tangential migration; spinal cord patterning; hippocampus development; activation of CREB transcription factor; neuron migration; ventral spinal cord development; response to pain; proteolysis; positive regulation of synaptic transmission, glutamatergic; positive regulation of small GTPase mediated signal transduction; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; glial cell differentiation; long-term memory; positive regulation of protein kinase activity; dendrite development; brain development; cell adhesion; associative learning; positive regulation of TOR signaling pathway; layer formation in the cerebral cortex; regulation of behavior

Disease: Epilepsy, Familial Temporal Lobe, 7; Lissencephaly 2; Epilepsy, Familial Temporal Lobe, 1

Research Articles on RL

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Product Notes

The Rat RL reln (Catalog #AAA2019179) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2019179 ELISA Kit recognizes Rat RL. It is sometimes possible for the material contained within the vial of "Reelin (RL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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