Human Reelin ELISA Kit | RELN elisa kit

Human Reelin ELISA Kit

Cat. Num. AAA760496

• Gene Names: RELN; RL; ETL7; LIS2; PRO1598
• Reactivity: Human
• Synonyms: Reelin; Human Reelin ELISA Kit; RELN (Reelin)/Reeler/RL; RELN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RELN. No significant cross-reactivity or interference between RELN and analogues was observed.
• Sequence Length: 3460

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for RELN elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1743885
• NCBI GeneID: 5649
• Molecular Weight: 384,093 Da
• NCBI Official Full Name: reelin
• NCBI Official Synonym Full Names: reelin
• NCBI Official Symbol: RELN
• NCBI Official Synonym Symbols: RL; ETL7; LIS2; PRO1598
• NCBI Protein Information: reelin
• UniProt Protein Name: Reelin
• Protein Family: Reelin
• UniProt Gene Name: RELN
• UniProt Entry Name: RELN_HUMAN

NCBI Description

This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

reelin: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2); also known as lissencephaly with cerebellar hypoplasia or Norman-Roberts syndrome. LIS2 is a classic type lissencephaly associated with abnormalities of the cerebellum, hippocampus and brainstem. Individuals with LIS2 are severely ataxic, mentally retarded and suffer from epilepsy. Belongs to the reelin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell adhesion; Protease; Cell development/differentiation; EC 3.4.21.-; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: cytoplasm; dendrite; extracellular space; proteinaceous extracellular matrix

Molecular Function: metal ion binding; protein serine/threonine/tyrosine kinase activity; serine-type peptidase activity

Biological Process: activation of CREB transcription factor; associative learning; axon guidance; brain development; cell adhesion; cellular morphogenesis during differentiation; central nervous system development; cerebral cortex tangential migration; dendrite development; glial cell differentiation; hippocampus development; layer formation in the cerebral cortex; long-term memory; neuron migration; peptidyl-tyrosine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase activity; positive regulation of small GTPase mediated signal transduction; positive regulation of synaptic transmission, glutamatergic; positive regulation of TOR signaling pathway; proteolysis; regulation of behavior; regulation of synaptic transmission; response to pain; spinal cord patterning; ventral spinal cord development

Disease: Epilepsy, Familial Temporal Lobe, 1; Epilepsy, Familial Temporal Lobe, 7; Lissencephaly 2