Proteasome 26S Subunit, Non ATPase 2 (PSMD2) Recombinant Protein | PSMD2 recombinant protein

Recombinant Proteasome 26S Subunit, Non ATPase 2 (PSMD2)

Cat. Num. AAA2018561

• Gene Names: PSMD2; S2; P97; RPN1; TRAP2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Proteasome 26S Subunit; Non ATPase 2 (PSMD2); Recombinant Proteasome 26S Subunit; PSMD2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DMGAHQ GVAVLGIALI AMGEEIGAEM ALRTFGHLLR YGEPTLRRAV PLALALISVS NPRLNILDTL SKFSHDADPE VSYNSIFAMG MVGSGTNNAR LAAMLRQLAQ YHAKDPNNLF MVRLAQGLTH LGKGTLTLCP YHSDRQLMSQ VAVAGLLTVL VSFLDVRNII LGKSHYVLYG LVAAMQPRML VTFDEELRPL PVSVRVGQAV DVVGQAGKPK TITGFQTHTT PV
• Sequence Length: 778

• Applicable Applications for PSMD2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asp645~Val872 (Accession # Q13200) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 523425734
• NCBI GeneID: 5708
• NCBI Accession #: NP_001265637.1
• NCBI GenBank Nucleotide #: NM_001278708.1
• UniProt Accession #: Q13200; Q12932; Q15321; Q53XQ4; Q96I12
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 2 isoform 2
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
• NCBI Official Symbol: PSMD2
• NCBI Official Synonym Symbols: S2; P97; RPN1; TRAP2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 2; 55.11 protein; protein 55.11; TNFR-associated protein 2; tumor necrosis factor type 1 receptor-associated protein 2
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 2
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD2
• UniProt Synonym Gene Names: TRAP2
• UniProt Entry Name: PSMD2_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

PSMD2: Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S2 family.

Protein type: Cell cycle regulation; Proteasome complex

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: nucleoplasm; proteasome complex; membrane; proteasome regulatory particle; cytosol

Molecular Function: protein binding; enzyme regulator activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis