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Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse EGR4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Early Growth Response Protein 4 (EGR4) Polyclonal Antibody | anti-EGR4 antibody
Polyclonal Antibody to Early Growth Response Protein 4 (EGR4)
Gene Names
Egr4; NGFIC; NGF1-C; NGFI-C; pAT133
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Early Growth Response Protein 4 (EGR4); Polyclonal Antibody; Polyclonal Antibody to Early Growth Response Protein 4 (EGR4); anti-EGR4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against EGR4. It has been selected for its ability to recognize EGR4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AVGTKVEDLL SISCPAELPG PAARLYQAGA YDTFSLAPGD LGEGTEGLPA LLTPPGGEGG SGGEGGEFLA APPAQLSPLG LRGAATADFS KPLVADLPGG SGVAAPSSPA ASFPAAKARR KGRRGGKCSA RCFCPRPHVK AFACPVESCV RSFARSDELN RHLRIHTGHK PFQCRICLRN FSRSDHLTTH VRTHTGEKPF
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AVGTKVEDLL SISCPAELPG PAARLYQAGA YDTFSLAPGD LGEGTEGLPA LLTPPGGEGG SGGEGGEFLA APPAQLSPLG LRGAATADFS KPLVADLPGG SGVAAPSSPA ASFPAAKARR KGRRGGKCSA RCFCPRPHVK AFACPVESCV RSFARSDELN RHLRIHTGHK PFQCRICLRN FSRSDHLTTH VRTHTGEKPF
Sequence Length
589
Applicable Applications for anti-EGR4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant EGR4 (Ala231~Ser474) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051439
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse EGR4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse EGR4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,554 Da
NCBI Official Full Name
early growth response protein 4
NCBI Official Synonym Full Names
early growth response 4
NCBI Official Symbol
Egr4
NCBI Official Synonym Symbols
NGFIC; NGF1-C; NGFI-C; pAT133
NCBI Protein Information
early growth response protein 4
UniProt Protein Name
Early growth response protein 4
Protein Family
UniProt Gene Name
Egr4
UniProt Synonym Gene Names
EGR-4
Uniprot Description
EGR4: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation (By similarity)
Protein type: C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 6 C3|6 37.48 cM
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding; nucleic acid binding; sequence-specific DNA binding
Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on EGR4
Similar Products
Product Notes
The EGR4 egr4 (Catalog #AAA2014829) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Early Growth Response Protein 4 (EGR4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Early Growth Response Protein 4 (EGR4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the EGR4 egr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AVGTKVEDLL SISCPAELPG PAARLYQAGA YDTFSLAPGD LGEGTEGLPA LLTPPGGEGG SGGEGGEFLA APPAQLSPLG LRGAATADFS KPLVADLPGG SGVAAPSSPA ASFPAAKARR KGRRGGKCSA RCFCPRPHVK AFACPVESCV RSFARSDELN RHLRIHTGHK PFQCRICLRN FSRSDHLTTH VRTHTGEKPF. It is sometimes possible for the material contained within the vial of "Early Growth Response Protein 4 (EGR4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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