Western Blot (WB)
(Western Blot: Sample: Recombinant KLF4, Mouse.)
Rabbit Kruppel Like Factor 4, Gut (KLF4) Polyclonal Antibody | anti-KLF4 antibody
Polyclonal Antibody to Kruppel Like Factor 4, Gut (KLF4)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant KLF4, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant KLF4, Mouse.)
Western Blot (WB)
(Western Blot:Sample: Mouse Stomach lysate Primary Ab: 1ug/ml Rabbit Anti-Mouse KLF4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047 ))
Western Blot (WB)
(Western Blot:Sample: Mouse Stomach lysate Primary Ab: 1ug/ml Rabbit Anti-Mouse KLF4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047 ))
NCBI and Uniprot Product Information
Uniprot Description
KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 4 B3|4 29.76 cM
Cellular Component: chromatin; cytoplasm; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex
Molecular Function: beta-catenin binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; histone deacetylase binding; metal ion binding; nucleic acid binding; phosphoinositide 3-kinase regulator activity; protein binding; sequence-specific DNA binding; transcription factor binding
Biological Process: cell differentiation; epidermal cell differentiation; epidermis morphogenesis; fat cell differentiation; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of heterotypic cell-cell adhesion; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of NF-kappaB transcription factor activity; negative regulation of phosphatidylinositol 3-kinase signaling; negative regulation of protein kinase B signaling cascade; negative regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of hemoglobin biosynthetic process; positive regulation of nitric oxide biosynthetic process; positive regulation of protein metabolic process; positive regulation of telomerase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; post-embryonic camera-type eye development; post-embryonic hemopoiesis; regulation of axon regeneration; regulation of cell differentiation; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to organic substance; response to retinoic acid; stem cell maintenance; transcription from RNA polymerase II promoter; transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin
Research Articles on KLF4
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Product Notes
The KLF4 klf4 (Catalog #AAA2014661) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Kruppel Like Factor 4, Gut (KLF4) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Kruppel Like Factor 4, Gut (KLF4) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the KLF4 klf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kruppel Like Factor 4, Gut (KLF4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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