Human LIM And SH3 Protein 1 (LASP1) ELISA Kit | LASP1 elisa kit

Human LIM And SH3 Protein 1 (LASP1) ELISA Kit

Cat. Num. AAA2022735

• Gene Names: LASP1; MLN50; Lasp-1
• Reactivity: Human
• Synonyms: LIM And SH3 Protein 1 (LASP1); Human LIM And SH3 Protein 1 (LASP1) ELISA Kit; MLN50; Metastatic lymph node gene 50 protein; LASP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LASP1. No significant cross-reactivity or interference between LASP1 and analogues was observed.
• Sequence Length: 205

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.062ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LASP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LASP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LASP1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LASP1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LASP1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LASP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LASP1 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 5453710
• NCBI GeneID: 3927
• NCBI Accession #: NP_006139.1
• NCBI GenBank Nucleotide #: NM_006148.3
• UniProt Accession #: Q14847; Q96ED2; Q96IG0; B4DGQ0
• Molecular Weight: 23,181 Da
• NCBI Official Full Name: LIM and SH3 domain protein 1 isoform a
• NCBI Official Synonym Full Names: LIM and SH3 protein 1
• NCBI Official Symbol: LASP1
• NCBI Official Synonym Symbols: MLN50; Lasp-1
• NCBI Protein Information: LIM and SH3 domain protein 1
• UniProt Protein Name: LIM and SH3 domain protein 1
• Protein Family: LIM and SH3 domain protein
• UniProt Gene Name: LASP1
• UniProt Synonym Gene Names: MLN50; LASP-1; MLN 50
• UniProt Entry Name: LASP1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]

Uniprot Description

Lasp-1: a member of the LIM protein subfamily, characterized by a LIM motif and SH3 domain. Functions as an actin-binding protein, possibly in cytoskeletal organization.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11-q21.3

Cellular Component: cortical actin cytoskeleton; focal adhesion

Molecular Function: actin filament binding; protein binding; SH3/SH2 adaptor activity; zinc ion binding; ion transmembrane transporter activity

Biological Process: positive regulation of signal transduction; ion transport