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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Cytochrome P450 26A1 (CYP26A1) Polyclonal Antibody | anti-CYP26A1 antibody

Polyclonal Antibody to Cytochrome P450 26A1 (CYP26A1)

Gene Names
Cyp26a1; RAH; Cyp26; P450RA; P450RAI
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Cytochrome P450 26A1 (CYP26A1); Polyclonal Antibody; Polyclonal Antibody to Cytochrome P450 26A1 (CYP26A1); anti-CYP26A1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CYP26A1. It has been selected for its ability to recognize CYP26A1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- N IRAKIRRLQA TEPDGGCKDA LQLLIEHSWE RGERLDMQAL KQSSTELLFG GHETTASAAT SLITYLGLYP HVLQKVREEI KSKGLLCKSN QDNKLDMETL EQLKYIGCVI KETLRLNPPV PGGFRVALKT FELNGYQIPK GWNVIYSICD THDVADIFTN KEEFNPDRFI VPHPEDASRF SFIPFGGGLR SCVGKEFAKI
Sequence Length
497
Applicable Applications for anti-CYP26A1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CYP26A1 (Asn250~Gly495) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2071793
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded brain tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded brain tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Kidney Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,178 Da
NCBI Official Full Name
cytochrome P450 26A1
NCBI Official Synonym Full Names
cytochrome P450, family 26, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp26a1
NCBI Official Synonym Symbols
RAH; Cyp26; P450RA; P450RAI
NCBI Protein Information
cytochrome P450 26A1
UniProt Protein Name
Cytochrome P450 26A1
Protein Family
UniProt Gene Name
Cyp26a1
UniProt Synonym Gene Names
Cyp26; P450ra

Uniprot Description

CYP26A1: Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18- hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA. Belongs to the cytochrome P450 family.

Protein type: Cofactor and Vitamin Metabolism - retinol; EC 1.14.-.-; Endoplasmic reticulum; Oxidoreductase

Chromosomal Location of Human Ortholog: 19|19 C2

Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; membrane

Molecular Function: heme binding; iron ion binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; retinoic acid 4-hydroxylase activity; retinoic acid binding

Biological Process: anterior/posterior pattern specification; central nervous system development; neural crest cell development; retinoic acid metabolic process; retinoic acid receptor signaling pathway; sterol metabolic process; xenobiotic metabolic process

Research Articles on CYP26A1

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Product Notes

The CYP26A1 cyp26a1 (Catalog #AAA2013628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cytochrome P450 26A1 (CYP26A1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 26A1 (CYP26A1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CYP26A1 cyp26a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- N IRAKIRRLQA TEPDGGCKDA LQLLIEHSWE RGERLDMQAL KQSSTELLFG GHETTASAAT SLITYLGLYP HVLQKVREEI KSKGLLCKSN QDNKLDMETL EQLKYIGCVI KETLRLNPPV PGGFRVALKT FELNGYQIPK GWNVIYSICD THDVADIFTN KEEFNPDRFI VPHPEDASRF SFIPFGGGLR SCVGKEFAKI. It is sometimes possible for the material contained within the vial of "Cytochrome P450 26A1 (CYP26A1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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