Cytochrome P450 26A1 (CYP26A1) Recombinant Protein | CYP26A1 recombinant protein

Recombinant Cytochrome P450 26A1 (CYP26A1)

Cat. Num. AAA2018386

• Gene Names: Cyp26a1; RAH; Cyp26; P450RA; P450RAI
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cytochrome P450 26A1 (CYP26A1); Recombinant Cytochrome P450 26A1 (CYP26A1); CYP26A1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- N IRAKIRRLQA TEPDGGCKDA LQLLIEHSWE RGERLDMQAL KQSSTELLFG GHETTASAAT SLITYLGLYP HVLQKVREEI KSKGLLCKSN QDNKLDMETL EQLKYIGCVI KETLRLNPPV PGGFRVALKT FELNGYQIPK GWNVIYSICD THDVADIFTN KEEFNPDRFI VPHPEDASRF SFIPFGGGLR SCVGKEFAKILLKIFTVELA RHCDWQLLNG PPTMKTSPTV YPVDNLPARF TYFQG
• Sequence Length: 497

• Applicable Applications for CYP26A1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Asn250~Gly495 (Accession # O55127) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 178057351
• NCBI GeneID: 13082
• NCBI Accession #: NP_031837.2
• NCBI GenBank Nucleotide #: NM_007811.2
• UniProt Accession #: O55127; Q9R1F4
• Molecular Weight: 31.6kDa
• NCBI Official Full Name: cytochrome P450 26A1
• NCBI Official Synonym Full Names: cytochrome P450, family 26, subfamily a, polypeptide 1
• NCBI Official Symbol: Cyp26a1
• NCBI Official Synonym Symbols: RAH; Cyp26; P450RA; P450RAI
• NCBI Protein Information: cytochrome P450 26A1; cytochrome P450RAI; retinoic acid hydrolase; retinoic acid 4-hydroxylase; cytochrome P450, 26, retinoic acid A1; retinoic acid-metabolizing cytochrome
• UniProt Protein Name: Cytochrome P450 26A1
• Protein Family: Cytochrome
• UniProt Gene Name: Cyp26a1
• UniProt Synonym Gene Names: Cyp26; P450ra
• UniProt Entry Name: CP26A_MOUSE

Uniprot Description

CYP26A1: Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18- hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; EC 1.14.-.-; Endoplasmic reticulum

Cellular Component: membrane; intracellular membrane-bound organelle; endoplasmic reticulum

Molecular Function: tert-butyl alcohol 2-monooxygenase activity; retinoic acid binding; methylsilanetriol hydroxylase activity; metal ion binding; retinoic acid 4-hydroxylase activity; alpha-pinene dehydrogenase activity; hydroxymethylsilanetriol oxidase activity; oxidoreductase activity; tri-n-butyltin dioxygenase activity; fluorene oxygenase activity; 2-hydroxyisobutyrate 3-monooxygenase activity; 4-nitrocatechol 4-monooxygenase activity; alpha-pinene monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; dimethylsilanediol hydroxylase activity; spheroidene monooxygenase activity; heme binding; mono-butyltin dioxygenase activity; ammonia monooxygenase activity; 4-chlorophenoxyacetate monooxygenase activity; methyl tertiary butyl ether 3-monooxygenase activity; monooxygenase activity; di-n-butyltin dioxygenase activity

Biological Process: retinoic acid receptor signaling pathway; anterior/posterior pattern formation; central nervous system development; neural crest cell development; retinoic acid metabolic process