Mouse Integrin Beta 3 (ITGb3) RTU ELISA Kit | ITGb3 rtu elisa kit

Mouse Integrin Beta 3 (ITGb3) ELISA Kit

Cat. Num. AAA4500526

• Gene Names: Itgb3; CD61; GP3A; INGRB3
• Reactivity: Mouse
• Synonyms: Integrin Beta 3 (ITGb3); Mouse Integrin Beta 3 (ITGb3) ELISA Kit; CD61; ITG-B3; GP3A; GP3-A; GpIIIa; Platelet Membrane Glycoprotein IIIa; ITGb3 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 787

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 13.5pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGb3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ITGb3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ITGb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITGb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ITGb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 160358856
• NCBI GeneID: 16416
• NCBI Accession #: NP_058060.2
• NCBI GenBank Nucleotide #: NM_016780.2
• UniProt Accession #: O54890; Q3TZC6
• Molecular Weight: 86,738 Da
• NCBI Official Full Name: integrin beta-3
• NCBI Official Synonym Full Names: integrin beta 3
• NCBI Official Symbol: Itgb3
• NCBI Official Synonym Symbols: CD61; GP3A; INGRB3
• NCBI Protein Information: integrin beta-3
• UniProt Protein Name: Integrin beta-3
• Protein Family: Integrin
• UniProt Gene Name: Itgb3
• UniProt Synonym Gene Names: GPIIIa

Uniprot Description

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surfaces. Fibrinogen binding enhances SELP expression in activated platelets (PubMed:19332769). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 ().