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SDS-Page

Phosphoinositide-3-Kinase Class-2-Beta Polypeptide (PIK3C2b) Recombinant Protein | PIK3C2b recombinant protein

Recombinant Phosphoinositide-3-Kinase Class-2-Beta Polypeptide (PIK3C2b)

Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Phosphoinositide-3-Kinase Class-2-Beta Polypeptide (PIK3C2b); Recombinant Phosphoinositide-3-Kinase Class-2-Beta Polypeptide (PIK3C2b); PIK3C2b recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- S PRYEFGSLRE EDQRKLKDIT QKESLYWLSD ADKKQLWEKR YYCHTEVSSL PLVLASAPSW EWACLPDIYA LLQQWTHMNH QDALGLLHAT FPDQEVRRMA VQWIGSLSDA ELLDYLPQLV QALKYECYLD SPLVRFLLKR AISDLRVTHY FFWLLKDSLK DSQFSIRYQY LLAALLCCCG KGLREEFNRQ CWLVNTLAKL AQQVREATPS ARQGILRAGL EEVRQFFALN GSCRL
Sequence Length
1621
Applicable Applications for PIK3C2b recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Ser790~Leu1025 (Accession # D3ZVF3) with two N-terminal Tags, His-tag and T7-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31.5kDa
NCBI Official Full Name
phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta
NCBI Official Symbol
Pik3c2b
NCBI Protein Information
phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta; nuclear cap binding protein subunit 2; phosphoinositide-3-kinase, class 2, beta polypeptide
UniProt Protein Name
Phosphoinositide-3-kinase, class 2, beta polypeptide (Predicted)
UniProt Gene Name
Pik3c2b
UniProt Synonym Gene Names
Pik3c2b_predicted
UniProt Entry Name
D3ZVF3_RAT

Uniprot Description

Sequence similarities: Contains 1 C2 domain. RuleBase RU003412Contains PI3K/PI4K domain. SAAS SAAS015433Contains PIK helical domain. SAAS SAAS018936

Research Articles on PIK3C2b

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Product Notes

The PIK3C2b pik3c2b (Catalog #AAA2012331) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phosphoinositide-3-Kinase Class-2-Beta Polypeptide (PIK3C2b) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the PIK3C2b pik3c2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- S PRYEFGSLRE EDQRKLKDIT QKESLYWLSD ADKKQLWEKR YYCHTEVSSL PLVLASAPSW EWACLPDIYA LLQQWTHMNH QDALGLLHAT FPDQEVRRMA VQWIGSLSDA ELLDYLPQLV QALKYECYLD SPLVRFLLKR AISDLRVTHY FFWLLKDSLK DSQFSIRYQY LLAALLCCCG KGLREEFNRQ CWLVNTLAKL AQQVREATPS ARQGILRAGL EEVRQFFALN GSCRL. It is sometimes possible for the material contained within the vial of "Phosphoinositide-3-Kinase Class-2-Beta Polypeptide (PIK3C2b), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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