Interleukin 1 Receptor Associated Kinase 2 (IRAK2) Recombinant Protein | IRAK2 recombinant protein

Recombinant Interleukin 1 Receptor Associated Kinase 2 (IRAK2)

Cat. Num. AAA2012226

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 1 Receptor Associated Kinase 2 (IRAK2); Recombinant Interleukin 1 Receptor Associated Kinase 2 (IRAK2); IRAK2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- DQSHRISEGT FADIYRGQRN GVAFAFKRLR EVTGSSPGSM DRFLQAEMQL CLRCCHPNIL PLLGFCTGRQ FHSLIYPYMA NGSLQDRLWA QGDSDMLSWP QRASICSGLL LAVEHLHSLD IIHSNVKSAN VLLDQHLNPK LAHPVAHPCP TNKKTKYTVM KTHLFQASAA YLPENFIRVG QLTKQVDIFS CGIVLAEVLT GIPAMDKDRS PVYLKDLLLS EIPSNTSSVH SRKTSMGKVV VKEICQKH
• Sequence Length: 624

• Applicable Applications for IRAK2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asp211~His458 (Accession # Q4QQS0) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 70794740
• NCBI GeneID: 362418
• NCBI Accession #: NP_001020593.1
• NCBI GenBank Nucleotide #: NM_001025422.1
• UniProt Accession #: Q4QQS0
• Molecular Weight: 29.2kDa
• NCBI Official Full Name: interleukin-1 receptor-associated kinase-like 2
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 2
• NCBI Official Symbol: Irak2
• NCBI Protein Information: interleukin-1 receptor-associated kinase-like 2; IRAK-2
• UniProt Protein Name: Interleukin-1 receptor-associated kinase-like 2
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: Irak2
• UniProt Synonym Gene Names: IRAK-2
• UniProt Entry Name: IRAK2_RAT

Uniprot Description

IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; IRAK family

Cellular Component: interleukin-1 receptor complex; cytoplasm; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein homodimerization activity; protein heterodimerization activity; ATP binding

Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of transcription factor activity; protein amino acid autophosphorylation; activation of NF-kappaB-inducing kinase; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; regulation of cytokine and chemokine mediated signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor