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Sequence Information

Selenoprotein P1, Plasma (SEPP1) Recombinant Protein | SEPP1 recombinant protein

Recombinant Selenoprotein P1, Plasma (SEPP1)

Gene Names
SEPP1; SeP; SELP
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Selenoprotein P1; Plasma (SEPP1); Recombinant Selenoprotein P1; SEPP1 recombinant protein
Ordering
For Research Use Only!
Host
E. coli
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5%Trehalose.
Concentration
Original Concentration: 550ug/mL (varies by lot)
Applicable Applications for SEPP1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Wetsern Blot (WB)
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Tyr60~Ser299
Tags
N-terminal His-Tag
Tissue Specificity
Liver, Kidney
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
7.7
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

Sequence Information

SDS-Page

SDS-Page

Testing Data

Testing Data

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 23.3kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions
NCBI Official Full Name
selenoprotein P isoform 1
NCBI Official Synonym Full Names
selenoprotein P, plasma, 1
NCBI Official Symbol
SEPP1
NCBI Official Synonym Symbols
SeP; SELP
NCBI Protein Information
selenoprotein P
UniProt Protein Name
Selenoprotein P
Protein Family
UniProt Gene Name
SEPP1
UniProt Synonym Gene Names
SELP; SeP
UniProt Entry Name
SEPP1_HUMAN

NCBI Description

This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SEPP1: Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. Belongs to the selenoprotein P family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding

Biological Process: sexual reproduction; locomotory behavior; response to oxidative stress; brain development; selenium metabolic process; post-embryonic development; growth

Research Articles on SEPP1

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Product Notes

The SEPP1 sepp1 (Catalog #AAA2012091) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Selenoprotein P1, Plasma (SEPP1) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Wetsern Blot (WB). (May be suitable for use in other assays to be determined by the end user.) . Researchers should empirically determine the suitability of the SEPP1 sepp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Selenoprotein P1, Plasma (SEPP1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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