Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) Recombinant Protein | CYR61 recombinant protein

Recombinant Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61)

Cat. Num. AAA2011986

• Gene Names: Cyr61; CCN1; Igfbp10; AI325051
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cysteine Rich Protein; Angiogenic Inducer 61 (CYR61); Recombinant Cysteine Rich Protein; CYR61 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-DDLLG LDASEVELTR NNELIAIGKG SSLKRLPVFG TEPRVLFNPL HAHGQKCIVQ TTSWSQCSKS CGTGISTRVT NDNPECRLVK ETRICEVRPC GQPVYSSLKK GKKCSKTKKS PEPVRFTYAG CSSVKKYRPK YCGSCVDGRC CTPLQTRTVK MRFRCEDGEM FSKNVMMIQS CKCNYNCPHP NEASFRLYSL FNDIHKFRD
• Sequence Length: 379

• Applicable Applications for CYR61 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Asp176~Asp379 (Accession # P18406) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.3
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753594
• NCBI GeneID: 16007
• NCBI Accession #: NP_034646.1
• NCBI GenBank Nucleotide #: NM_010516.2
• UniProt Accession #: P18406
• Molecular Weight: 26.8kDa
• NCBI Official Full Name: protein CYR61
• NCBI Official Synonym Full Names: cysteine rich protein 61
• NCBI Official Symbol: Cyr61
• NCBI Official Synonym Symbols: CCN1; Igfbp10; AI325051
• NCBI Protein Information: protein CYR61; 3CH61; IBP-10; IGFBP-10; intermediate early; CCN family member 1; IGF-binding protein 10; cysteine-rich angiogenic inducer 61; insulin-like growth factor binding protein 10; insulin-like growth factor-binding protein 10
• UniProt Protein Name: Protein CYR61
• Protein Family: Protein
• UniProt Gene Name: Cyr61
• UniProt Synonym Gene Names: Ccn1; Igfbp10; IBP-10; IGF-binding protein 10; IGFBP-10
• UniProt Entry Name: CYR61_MOUSE

Uniprot Description

CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; extracellular matrix binding; growth factor binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of caspase activity; chemotaxis; signal transduction; intussusceptive angiogenesis; osteoblast differentiation; positive regulation of osteoblast differentiation; cell-cell adhesion; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; regulation of cell growth; positive regulation of cell differentiation; cell adhesion; positive regulation of BMP signaling pathway; positive regulation of cell migration; negative regulation of apoptosis