Apolipoprotein A5 (APOA5) Recombinant Protein | APOA5 recombinant protein

Recombinant Apolipoprotein A5 (APOA5)

Cat. Num. AAA2011902

• Gene Names: Apoa5; apo-AV; apoA-V
• Reactivity: Rattus norvegicus (Rat)
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Apolipoprotein A5 (APOA5); Recombinant Apolipoprotein A5 (APOA5); APOA5 recombinant protein

• Host: E Coli
• Reactivity: Rattus norvegicus (Rat)
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-QD MQSRVLHHTD RVKELFHPYA ERLVTGIGHH VQELHRSVAP HAVASPARLS RCVQTLSHKL TRKAKDLHTS IQRNLDQLRD ELSTFIRVST DGADNRDSLD PQALSDEVRQ RLQAFRHDTY LQIAAFTQAI DQETEEIQHQ LAPPPPSHSA FAPELGHSDS NKALSRLQSR LDDLWEDIAY GLHDQGHSQN NPEGHSG
• Sequence Length: 367

• Applicable Applications for APOA5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Expression System: Prokaryotic expression
• Residues: Gln169~Gly367 (Accession # Q9QUH3) with N-terminal His-Tag
• Predicted isoelectric point: 6.2
• Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in ddH2O.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 471270246
• NCBI GeneID: 140638
• NCBI Accession #: NP_001264193.1
• NCBI GenBank Nucleotide #: NM_001277264.1
• UniProt Accession #: Q9QUH3; Q5FVT8
• Molecular Weight: 24.0kDa
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A-V
• NCBI Official Symbol: Apoa5
• NCBI Official Synonym Symbols: apo-AV; apoA-V
• NCBI Protein Information: apolipoprotein A-V; apolipoprotein A5; regeneration-associated protein 3
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa5
• UniProt Synonym Gene Names: Rap3; Apo-AV; ApoA-V
• UniProt Entry Name: APOA5_RAT

NCBI Description

member of the apolipoprotein family; expression increases during liver regeneration [RGD, Feb 2006]

Uniprot Description

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Lipid-binding; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; chylomicron; extracellular region

Molecular Function: heparin binding; enzyme binding; low-density lipoprotein receptor binding; cholesterol transporter activity; enzyme activator activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding

Biological Process: positive regulation of fatty acid biosynthetic process; organ regeneration; response to hormone stimulus; neurite regeneration; positive regulation of lipid catabolic process; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; phospholipid efflux; regulation of cholesterol absorption; acylglycerol homeostasis; tissue regeneration; triacylglycerol catabolic process; positive regulation of lipoprotein lipase activity