VAP-1/AOC3 Recombinant Protein | VAP-1 recombinant protein

VAP-1/AOC3

Cat. Num. AAA201189

• Gene Names: AOC3; HPAO; SSAO; VAP1; VAP-1
• Applications: SDS-Page
• Purity: >90% by SDS - PAGE
• Synonyms: VAP-1/AOC3; VAP-1; AOC3; HPAO; VAP1; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 27-763aa
• Sequence: GRGGDGG EPSQLPHCPS VSPSAQPWTH PGQSQLFADL SREELTAVMR FLTQRLGPGL VDAAQARPSD NCVFSVELQL PPKAAALAHL DRGSPPPARE ALAIVFFGRQ PQPNVSELVV GPLPHPSYMR DVTVERHGGP LPYHRRPVLF QEYLDIDQMI FNRELPQASG LLHHCCFYKH RGRNLVTMTT APRGLQSGDR ATWFGLYYNI SGAGFFLHHV GLELLVNHKA LDPARWTIQK VFYQGRYYDS LAQLEAQFEA GLVNVVLIPD NGTGGSWSLK SPVPPGPAPP LQFYPQGPRF SVQGSRVASS LWTFSFGLGA FSGPRIFDVR FQGERLVYEI SLQEALAIYG GNSPAAMTTR YVDGGFGMGK YTTPLTRGVD CPYLATYVDW HFLLESQAPK TIRDAFCVFE QNQGLPLRRH HSDLYSHYFG GLAETVLVVR SMSTLLNYDY VWDTVFHPSG AIEIRFYATG YISSAFLFGA TGKYGNQVSE HTLGTVHTHS AHFKVDLDVA GLENWVWAED MVFVPMAVPW SPEHQLQRLQ VTRKLLEMEE QAAFLVGSAT PRYLYLASNH SNKWGHPRGY RIQMLSFAGE PLPQNSSMAR GFSWERYQLA VTQRKEEEPS SSSVFNQNDP WAPTVDFSDF INNETIAGKD LVAWVTAGFL HIPHAEDIPN TVTVGNGVGF FLRPYNFFDE DPSFYSADSI YFRGDQDAGA CEVNPLACLP QAAACAPDLP AFSHGGFSHN
• Sequence Length: 763

• Applicable Applications for VAP-1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag: His-Tag
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Predicted N-Terminal: Ala
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for VAP-1 recombinant protein

VAP-1, also known as membrane primary amine oxidase isoform 1, a copper amine oxidase with a topaquinone cofactor. It is a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. This protein plays a role in adipogenesis. It catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. Recombinant human VAP-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for VAP-1 recombinant protein

Neuroscience

References

Hsia LT., et al. (2016) Proc Natl Acad Sci U S A. 113:E2162-71; Trivedi PJ., et al. (2018) Gut. 67:1135-1145

NCBI and Uniprot Product Information

• NCBI GI #: 4502119
• NCBI GeneID: 8639
• NCBI Accession #: NP_003725
• NCBI GenBank Nucleotide #: NP_003725.1
• UniProt Accession #: Q16853
• Molecular Weight: 82.8kDa (746aa)
• NCBI Official Full Name: membrane primary amine oxidase isoform 1
• NCBI Official Synonym Full Names: amine oxidase copper containing 3
• NCBI Official Symbol: AOC3
• NCBI Official Synonym Symbols: HPAO; SSAO; VAP1; VAP-1
• NCBI Protein Information: membrane primary amine oxidase
• UniProt Protein Name: Membrane primary amine oxidase
• Protein Family: Vesicle-associated protein
• UniProt Gene Name: AOC3
• UniProt Synonym Gene Names: VAP1; SSAO; VAP-1
• UniProt Entry Name: AOC3_HUMAN

NCBI Description

This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Cell adhesion; Oxidoreductase; Amino Acid Metabolism - phenylalanine; Other Amino Acids Metabolism - beta-alanine; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - glycine, serine and threonine; EC 1.4.3.21

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cell surface; microvillus; cytoplasm; plasma membrane; integral to membrane

Molecular Function: amine oxidase activity; protein binding; protein homodimerization activity; copper ion binding; protein heterodimerization activity; cation channel activity; calcium ion binding; quinone binding

Biological Process: response to antibiotic; amine metabolic process; cell adhesion; inflammatory response; cation transport