Keratin 10 (KRT10) Recombinant Protein | KRT10 recombinant protein

Recombinant Keratin 10 (KRT10)

Cat. Num. AAA2011283

• Gene Names: Krt10; Ka10
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Keratin 10 (KRT10); Recombinant Keratin 10 (KRT10); KRT10 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-LNNM RNQYEQLAEK NRKDAEAWFN EKSKELTTEI DSNIEQMSSH KSEITELRRT VQGLEIELQS QLALKQSLEA SLAETEGRYC VQLSQIQSQI SALEEQLQQI RAETECQNAE YQQLLDIKTR LENEIQTYRS LLEGE
• Sequence Length: 526

• Applicable Applications for KRT10 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu317~Glu455 (Accession # Q6IFW6) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 57012436
• NCBI GeneID: 450225
• NCBI Accession #: NP_001008804.1
• NCBI GenBank Nucleotide #: NM_001008804.1
• UniProt Accession #: Q6IFW6
• Molecular Weight: 21.9kDa
• NCBI Official Full Name: keratin, type I cytoskeletal 10
• NCBI Official Synonym Full Names: keratin 10
• NCBI Official Symbol: Krt10
• NCBI Official Synonym Symbols: Ka10
• NCBI Protein Information: keratin, type I cytoskeletal 10; K10; CK-10; keratin-10; cytokeratin-10; type I keratin KA10
• UniProt Protein Name: Keratin, type I cytoskeletal 10
• Protein Family: Keratin
• UniProt Gene Name: Krt10
• UniProt Synonym Gene Names: Ka10; CK-10; K10
• UniProt Entry Name: K1C10_RAT

Uniprot Description

K10: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K1.

Protein type: Cytoskeletal

Cellular Component: extracellular space; membrane; keratin filament; cytoplasm; nucleus

Molecular Function: structural molecule activity

Biological Process: keratinocyte differentiation; epithelial cell differentiation