Cardiac Troponin I Recombinant Protein | cTnl recombinant protein

Recombinant Cardiac Troponin I

Cat. Num. AAA201100

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Cardiac Troponin I; Recombinant Cardiac Troponin I; Troponin I3; CMD1FF; CMD2A; CMH7; RCM1; TNNC1; cTnl recombinant protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In 20mM Tris-HCl Buffer (pH7.5) Containing 30% Glycerol, 0.1M NaCl, 0.1mM PMSF, 1mM DTT.
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 1-210aa
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADGSSD AAREPRPAPA PIRRRSSNYR AYATEPHAKK KSKISASRKL QLKTLLLQIA KQELEREAEE RRGEKGRALS TRCQPLELAG LGFAELQDLC RQLHARVDKV DEERYDIEAK VTKNITEIAD LTQKIFDLRG KFKRPTLRRV RISADAMMQA LLGARAKESL DLRAHLKQVK KEDTEKENRE VGDWRKNIDA LSGMEGRKKK FES
• Sequence Length: 210

• Applicable Applications for cTnl recombinant protein: SDS-PAGE
• Species: Human
• Tag: His-Tag
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for cTnl recombinant protein

TNNI3, also known as Troponin I3, is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TNNI3 is responsible for inhibition of actomyosin ATPase activity. Therefore, TNNI3 is a key regulatory protein in cardiac muscle contraction and relaxation cycle. Recently, specific missense mutations of the TNNI3 gene have been shown to cause familial hypertrophic cardiomyopathy. Recombinant human TNNI3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography.

Product Categories/Family for cTnl recombinant protein

Cardiovascular

References

DW Sohn., et al. (2017) Korean Circ J. 47(3):413-417. Elliot K., et al. (2000) J Biol Chem. 275(29):22069-74.

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NP_000354.4
• UniProt Accession #: P19429
• Molecular Weight: 26.4 kDa (233aa) confirmed by MALDI-TOF
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I3, cardiac type
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1
• UniProt Entry Name: TNNI3_HUMAN

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding

Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a