Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Recombinant Protein | ROR1 recombinant protein

Recombinant Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1)

Cat. Num. AAA2010915

• Gene Names: Ror1; mRor1; Ntrkr1; 2810404D04Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1); Recombinant Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1); ROR1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-L SAVRFMEELG ECTFGKIYKG HLYLPGMDHA QLVAIKTLKD YNNPQQWTEF QQEASLMAEL HHPNIVCLLG AVTQEQPVCM LFEYMNQGDL HEFLIMRSPH SDVGCSSDED GTVKSSLDHG DFLHIAIQIA AGMEYLSSHF FVHKDLAARN ILIGEQLHVK ISDLGLSREI YSADYYRVQS KSSLPIRWMP PEAIMYGKFS SDSDIWSFGV VLWEIFSFGL QPYYGFSNQE VIEMVRK
• Sequence Length: 937

• Applicable Applications for ROR1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu470~Lys707 (Accession # Q9Z139) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31560493
• NCBI GeneID: 26563
• NCBI Accession #: NP_038873.2
• NCBI GenBank Nucleotide #: NM_013845.4
• UniProt Accession #: Q9Z139; Q8BG10
• Molecular Weight: 31.0kDa
• NCBI Official Full Name: tyrosine-protein kinase transmembrane receptor ROR1
• NCBI Official Synonym Full Names: receptor tyrosine kinase-like orphan receptor 1
• NCBI Official Symbol: Ror1
• NCBI Official Synonym Symbols: mRor1; Ntrkr1; 2810404D04Rik
• NCBI Protein Information: tyrosine-protein kinase transmembrane receptor ROR1; neurotrophic tyrosine kinase, receptor related 1; neurotrophic tyrosine kinase, receptor-related 1
• UniProt Protein Name: Tyrosine-protein kinase transmembrane receptor ROR1
• Protein Family: Inactive tyrosine-protein kinase transmembrane receptor
• UniProt Gene Name: Ror1
• UniProt Synonym Gene Names: Ntrkr1; mROR1
• UniProt Entry Name: ROR1_MOUSE

NCBI Description

This gene encodes a receptor tyrosine kinase that has been implicated in nervous system development, specifically in the maintenance of neural progenitor cell fate, neurite extension and synapse formation. The encoded protein, likely a pseudokinase that lacks catalytic activity, may also regulate adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

ROR1: Tyrosine-protein kinase receptor whose role is not yet clear. Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Kinase, protein; TK group; Ror family

Cellular Component: membrane; integral to plasma membrane; integral to membrane; receptor complex

Molecular Function: transferase activity; Wnt-protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; signal transduction; protein amino acid phosphorylation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway