Lactoferrin (LTF) Recombinant Protein | LTF recombinant protein

Recombinant Lactoferrin (LTF)

Cat. Num. AAA2010495

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lactoferrin (LTF); Recombinant Lactoferrin (LTF); LTF recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-VRWCAI SLPEWSKCYQ WQRRMRKLGA PSITCIRRTS ALECIRAIAG KNADAVTLDS GMVFEAGLDP YKLRPVAAEI YGTEKSPQTH YYAVAVVKKG SNFQLDQLQG QKSCHMGLGR SAGWNIPVGI LRPFLSWTES AEPLQGAVAR FFSASCVPCV DGKAYPNLCQ LCKGVGENKC ACSSQEPYFG YSGAFKCLQD GAGDVAFVKE TTVFENLPEK ADRDQYELLC LNNTRAPVDA FKECHLAQVP SHAVVARSVD GKENLIWELL RKAQEKFGKN KSQSFQLFGS PEGRRDLLFK DSALGFVRIP SKVDSALYLG SRYLTALKNL RE
• Sequence Length: 708

• Applicable Applications for LTF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Capra hircus; Caprine (Goat)
• Expression System: Prokaryotic expression
• Residues: Val25~Glu352 (Accession # Q29477) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 550822103
• NCBI GeneID: 100861194
• NCBI Accession #: NP_001272477.1
• NCBI GenBank Nucleotide #: NM_001285548.1
• UniProt Accession #: Q29477; Q29479
• Molecular Weight: 37.9kDa
• NCBI Official Full Name: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Protein Information: lactotransferrin; lactoferrin
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: Lactoferrin
• UniProt Entry Name: TRFL_CAPHI

Uniprot Description

Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity. Antimicrobial properties may include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. The most effective inhibitory activity is seen against E.coli and P.aeruginosa. Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. Interferes with the lipopolysaccharide (LPS)-stimulated TLR4 signaling, but cannot directly stimulate the TLR4 signaling pathway and subsequent NF-kappa-B activation

By similarity.The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity

By similarity.

Catalytic activity: Preferential at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin.

Subunit structure: Monomer

By similarity. Found in a complex with LTF, CLU, EPPIN and SEMG1

By similarity.

Subcellular location: Secreted. Cytoplasmic granule

By similarity. Note: Secreted into most exocrine fluids by various endothelial cells. Stored in the secondary granules of neutrophils

By similarity.

Developmental stage: Expressed in mammary glands at various stages of development, with weak expression detected in virgin goats and during pregnancy and lactation and high expression detected at the stage of involution. Ref.2

Sequence similarities: Belongs to the transferrin family.Contains 2 transferrin-like domains.