Hyaluronan Binding Protein 1 (HABP1) Recombinant Protein | HABP1 recombinant protein

Recombinant Hyaluronan Binding Protein 1 (HABP1)

Cat. Num. AAA2010081

• Gene Names: C1qbp; P32; HABP1; gC1qBP; AA407365; AA986492; D11Wsu182e
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Hyaluronan Binding Protein 1 (HABP1); Recombinant Hyaluronan Binding Protein 1 (HABP1); HABP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-LHTEGDKAFV EFLTDEIKEE KKIQKHKSLP KMSGDWELEV NGTEAKLLRK VAGEKITVTF NINNSIPPTF DGEEEPSQGQ KAEEQEPERT STPNFVVEVT KTDGKKTLVL DCHYPEDEIG HEDEAESDIF SIKEVSFQAT GDSEWRDTNY TLNTDSLDWA LYDHLMDFLA DRGVDNTFAD ELVELSTALE HQEYITFLED LKSFVKNQ
• Sequence Length: 278

• Applicable Applications for HABP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu71~Gln278 (Accession # O35658) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 3334247
• NCBI GeneID: 12261
• UniProt Accession #: O35658
• Molecular Weight: 25.0kDa
• NCBI Official Full Name: Complement component 1 Q subcomponent-binding protein, mitochondrial
• NCBI Official Synonym Full Names: complement component 1, q subcomponent binding protein
• NCBI Official Symbol: C1qbp
• NCBI Official Synonym Symbols: P32; HABP1; gC1qBP; AA407365; AA986492; D11Wsu182e
• NCBI Protein Information: complement component 1 Q subcomponent-binding protein, mitochondrial; GC1q-R protein; glycoprotein gC1qBP
• UniProt Protein Name: Complement component 1 Q subcomponent-binding protein, mitochondrial
• UniProt Gene Name: C1qbp
• UniProt Synonym Gene Names: Gc1qbp; C1qBP
• UniProt Entry Name: C1QBP_MOUSE

Uniprot Description

C1QBP: a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. Originally identified via its binding interactions with Splicing Factor (SF-2). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases, the tumor suppressor ARF, and multiple antigens of bacterial and viral origin. Overexpressed in a number of cancer cell types, and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis. Inhibits the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress. Binding to C1q inhibits C1. In complex with cytokeratin-1/KRT1 is a high affinty receptor for kininogen-1/HMWK. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. The cell surface form may require docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. Belongs to the MAM33 family.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Nucleolus; Mitochondrial

Cellular Component: extracellular space; cell surface; membrane; mitochondrion; mitochondrial matrix; cytoplasm; extracellular region; plasma membrane; cytosol; nucleus

Molecular Function: mRNA binding; protein binding; adrenergic receptor binding; protein kinase C binding; complement component C1q binding; hyaluronic acid binding; kininogen binding; transcription corepressor activity; transcription factor binding

Biological Process: phosphoinositide 3-kinase cascade; negative regulation of interleukin-12 production; positive regulation of cell adhesion; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; immune system process; RNA splicing; ribosome biogenesis and assembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of defense response to virus; positive regulation of protein kinase B signaling cascade; regulation of transcription, DNA-dependent; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of interferon-gamma production; regulation of complement activation; innate immune response; mRNA processing; complement activation, classical pathway; mature ribosome assembly