Caspase 1 (CASP1) Recombinant Protein | CASP1 recombinant protein

Recombinant Caspase 1 (CASP1)

Cat. Num. AAA2009994

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Caspase 1 (CASP1); Recombinant Caspase 1 (CASP1); CASP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-N PVKPASSEPR GSLKLCPPDI AQRLWKEKSA EIYPIMGKSI RTRLALIICN TEFENLPRRD GADVDIRDMK ILLEDLGYSV DVRENLTASD MAIELKAFAA RPEHKSSDST FLVLMSHGIQ AGICGKKYSE EVPDVLEVNT VFQILNTLNC PSLKDKPKVI IIQACRGEKQ GVVWIKD
• Sequence Length: 404

• Applicable Applications for CASP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Asn120~Asp297 (Accession # Q9N2I1) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 47523412
• NCBI GeneID: 397319
• NCBI Accession #: NP_999327.1
• NCBI GenBank Nucleotide #: NM_214162.1
• UniProt Accession #: Q9N2I1
• Molecular Weight: 21.4kDa
• NCBI Official Full Name: caspase-1
• NCBI Official Symbol: CASP1
• NCBI Protein Information: caspase-1; ICE; p45; CASP-1; IL-1BC; IL-1 beta-converting enzyme; interleukin-1 beta convertase; interleukin-1 beta converting enzyme; interleukin-1 beta-converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; CASP-1; IL-1BC; ICE
• UniProt Entry Name: CASP1_PIG

Uniprot Description

Function: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis

By similarity.

Catalytic activity: Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform whichthen has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18

By similarity.

Subcellular location: Cytoplasm.

Post-translational modification: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Sequence similarities: Belongs to the peptidase C14A family.Contains 1 CARD domain.