GDF-8 Recombinant Protein | MSTN recombinant protein

GDF-8 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122742

• Gene Names: Mstn; Cmpt; Gdf8
• Purity: >92% as determined by SDS-PAGE
• Synonyms: GDF-8; GDF-8 Protein; Mouse; Recombinant (hFc Tag); Mouse GDF-8/Myostatin/MSTN Protein (Fc Tag); Cmpt Protein; Gdf8 Protein; myostatin; MSTN recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp268-Ser376

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 20
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mature form of mouse MSTN (NP_034964.1)(Asp 268-Ser 376) was fused with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MSTN recombinant protein

Background: GDF-8/Myostatin/MSTN is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF-8/Myostatin/MSTN is highly expressed in skeletal muscle, and myostatin loss-of-function leads to doubling of skeletal muscle mass. Experiments in mice have improved that GDF-8/Myostatin/MSTN is a key regulator of mesenchymal stem cell proliferation and differentiation, and mice lacking Myostatin encoding gene show decreased body fat and a generalized increase in bone density and strength. The increase in bone density is observed in most anatomical regions, including the limbs, spine, and jaw, and myostatin inhibitors have been observed to significantly increase bone formation. GDF-8/Myostatin/MSTN is also expressed in the early phases of fracture healing, and GDF-8/Myostatin/MSTN deficiency leads to increased fracture callus size and strength. Together, these data suggest that GDF-8/Myostatin/MSTN has direct effects on the proliferation and differentiation of osteoprogenitor cells and that GDF-8/Myostatin/MSTN antagonists and inhibitors are likely to enhance both muscle mass and bone strength.

References

Elkasrawy MN, et al. (2010) Myostatin (GDF-8) as a key factor linking muscle mass and bone structure. J Musculoskelet Neuronal Interact. 10(1): 56-63.Kambadur R, et al. (1997) Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle. Genome Res. 7 (9): 910-6.McPherron AC, et al. (1997) Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature. 387 (6628): 83-90.

NCBI and Uniprot Product Information

• NCBI GI #: 6754752
• NCBI GeneID: 17700
• NCBI Accession #: NP_034964.1
• NCBI GenBank Nucleotide #: NM_010834.3
• UniProt Accession #: O08689
• Molecular Weight: 42,921 Da
• NCBI Official Full Name: growth/differentiation factor 8 preproprotein
• NCBI Official Synonym Full Names: myostatin
• NCBI Official Symbol: Mstn
• NCBI Official Synonym Symbols: Cmpt; Gdf8
• NCBI Protein Information: growth/differentiation factor 8
• UniProt Protein Name: Growth/differentiation factor 8
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: Mstn
• UniProt Synonym Gene Names: Gdf8; GDF-8

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Homozygous knockout mice for this gene exhibit increased muscle mass and bone density, and reduced adiposity. [provided by RefSeq, Jul 2016]

Uniprot Description

Acts specifically as a negative regulator of skeletal muscle growth.