Rabbit anti-Human, Mouse Survivin Polyclonal Antibody | anti-BIRC5 antibody

Survivin Polyclonal Antibody

Cat. Num. AAA3011744

• Gene Names: BIRC5; API4; EPR-1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Survivin; Polyclonal Antibody; Survivin Polyclonal Antibody; Apoptosis inhibitor survivin; Apoptosis inhibitor 4; BIRC5; API4; IAP4; anti-BIRC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Survivin polyclonal antibody detects endogenous levels of Survivin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 137

• Applicable Applications for anti-BIRC5 antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200; IP: 1:50-1:100
• Immunogen: Recombinant full length Human Survivin.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using BIRC5 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using BIRC5 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-BIRC5 antibody

Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino-terminal baculovirus IAP repeat (BIR) motif and a carboxy-terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FASmediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivinmicrotubule interactions occurs.

NCBI and Uniprot Product Information

• NCBI GI #: 59859880
• NCBI GeneID: 332
• NCBI Accession #: NP_001012270.1
• NCBI GenBank Nucleotide #: NP_001012270.1
• UniProt Accession #: O15392
• Molecular Weight: ~ 16kDa
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 5 isoform 2
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 5
• NCBI Official Symbol: BIRC5
• NCBI Official Synonym Symbols: API4; EPR-1
• NCBI Protein Information: baculoviral IAP repeat-containing protein 5
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 5
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC5
• UniProt Synonym Gene Names: API4; IAP4
• UniProt Entry Name: BIRC5_HUMAN

NCBI Description

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: centriole; nuclear chromosome; microtubule; spindle microtubule; cytoplasmic microtubule; cytoplasm; spindle; midbody; interphase microtubule organizing center; nucleus; cytosol; chromosome, pericentric region

Molecular Function: tubulin binding; identical protein binding; protein homodimerization activity; zinc ion binding; metal ion binding; microtubule binding; caspase inhibitor activity; ubiquitin-protein ligase activity; protein binding; enzyme binding; protein heterodimerization activity; chaperone binding; cysteine protease inhibitor activity; cofactor binding; Ran GTPase binding; cobalt ion binding

Biological Process: mitosis; transcription, DNA-dependent; spindle checkpoint; establishment of chromosome localization; regulation of signal transduction; cytokinesis; protein ubiquitination; negative regulation of caspase activity; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; protein complex localization; cell division; positive regulation of cell proliferation; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle; positive regulation of exit from mitosis; negative regulation of apoptosis