Motilin (MTL) Recombinant Protein | MTL recombinant protein

Recombinant Motilin (MTL)

Cat. Num. AAA2009916

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Motilin (MTL); Recombinant Motilin (MTL); MTL recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- FVPIF TYGELQRMQE KERNKGQKKS LSVWQRSGEE GPVDPAEPIR EEENEMIKLT APLEIGMRMN SRQLEKYPAT LEGLLSEMLP QHAAK
• Sequence Length: 114

• Applicable Applications for MTL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Phe26~Lys115 (Accession # P12872) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 92091580
• NCBI GeneID: 4295
• NCBI Accession #: NP_001035198.1
• NCBI GenBank Nucleotide #: NM_001040109.1
• UniProt Accession #: P12872; Q2M1L2; Q5T975; Q6NSY7; B7ZLR7; E9PDN2; J3KN51
• Molecular Weight: 11.9kDa
• NCBI Official Full Name: promotilin isoform 2 preproprotein
• NCBI Official Synonym Full Names: motilin
• NCBI Official Symbol: MLN
• NCBI Protein Information: promotilin; prepromotilin
• UniProt Protein Name: Promotilin
• UniProt Gene Name: MLN
• UniProt Synonym Gene Names: MAP
• UniProt Entry Name: MOTI_HUMAN

NCBI Description

This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

MLN: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle. Belongs to the motilin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling