Non Metastatic Cells 4, Protein NM23A Expressed In (NME4) Recombinant Protein | NME4 recombinant protein

Recombinant Non Metastatic Cells 4, Protein NM23A Expressed In (NME4)

Cat. Num. AAA2009851

• Gene Names: NME4; NDPK-D; NM23H4; nm23-H4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Non Metastatic Cells 4; Protein NM23A Expressed In (NME4); Recombinant Non Metastatic Cells 4; NME4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-TLVG MKMLQAPESV LAEHYQDLRR KPFYPALIRY MSSGPVVAMV WEGYNVVRAS RAMIGHTDSA EAAPGTIRGD FSVHISRNVI HASDSVEGAQ REIQLWFQSS ELVSWADGGQ HSSIH
• Sequence Length: 153

• Applicable Applications for NME4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Thr67~His185 (Accession # O00746) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 556695376
• NCBI GeneID: 4833
• NCBI Accession #: NP_001273362.1
• NCBI GenBank Nucleotide #: NM_001286433.1
• UniProt Accession #: O00746; Q5U0M9
• Molecular Weight: 17.0kDa
• NCBI Official Full Name: nucleoside diphosphate kinase, mitochondrial isoform b
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 4
• NCBI Official Symbol: NME4
• NCBI Official Synonym Symbols: NDPK-D; NM23H4; nm23-H4
• NCBI Protein Information: nucleoside diphosphate kinase, mitochondrial; NDK; NDPKD; NDP kinase D; NDP kinase, mitochondrial; nucleoside diphosphate kinase D; non-metastatic cells 4, protein expressed in
• UniProt Protein Name: Nucleoside diphosphate kinase, mitochondrial
• UniProt Gene Name: NME4
• UniProt Synonym Gene Names: NM23D; NDK; NDP kinase, mitochondrial; NDPKD
• UniProt Entry Name: NDKM_HUMAN

NCBI Description

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]

Uniprot Description

NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family.

Protein type: Nucleotide Metabolism - purine; EC 2.7.4.6; Mitochondrial; Kinase, nucleoside diphosphate; Nucleotide Metabolism - pyrimidine; Kinase, other; Other group; NDK family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol

Molecular Function: protein binding; metal ion binding; protein complex binding; nucleoside diphosphate kinase activity; ATP binding

Biological Process: regulation of apoptosis; GTP biosynthetic process; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside metabolic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process