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Spondin 2 (SPON2) Recombinant Protein | SPON2 recombinant protein
Recombinant Spondin 2 (SPON2)
Gene Names
SPON2; DIL1; DIL-1; MINDIN; M-SPONDIN
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Spondin 2 (SPON2); Recombinant Spondin 2 (SPON2); SPON2 recombinant protein
Host
E.coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 120ug/mL (varies by lot)
Sequence
SV HEVFSAPAVP SGTGQTSAEL EVQRRHSLVS FVVRIVPSPD WFVGVDSLDL CDGDRWREQA ALDLYPYDAG TDSGFTFSSP NFATIPQDTV TEITSSSPSH PANSFYYPRL KALPPIARVT LVRLRQSPRA FIPPAPVLPS RDNEIVDSAS VPETPLDCEV SLWSSWGLCG GHCGRLGTKS RTRYVRVQPA NNGSPCPELE EEAECVPDNC V
Applicable Applications for SPON2 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
Predicted Molecular Mass: 53.1kDa
Accurate Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions.
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Ser119~Val331
Tags
N-terminal His and GST Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
5.6
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
spondin-2
NCBI Official Synonym Full Names
spondin 2, extracellular matrix protein
NCBI Official Symbol
SPON2
NCBI Official Synonym Symbols
DIL1; DIL-1; MINDIN; M-SPONDIN
NCBI Protein Information
spondin-2; differentially expressed in cancerous and non-cancerous lung cells 1
UniProt Protein Name
Spondin-2
Protein Family
UniProt Gene Name
SPON2
UniProt Synonym Gene Names
DIL1; DIL-1
UniProt Entry Name
SPON2_HUMAN
Uniprot Description
SPON2: Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern- recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 4p16.3
Cellular Component: extracellular space; proteinaceous extracellular matrix
Molecular Function: lipopolysaccharide binding; metal ion binding; antigen binding
Biological Process: mast cell mediated immunity; axon guidance; induction of bacterial agglutination; innate immune response; positive regulation of interleukin-6 production; opsonization; cell adhesion; positive regulation of tumor necrosis factor production; defense response to virus; defense response to fungus
Research Articles on SPON2
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Product Notes
The SPON2 spon2 (Catalog #AAA2009592) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Spondin 2 (SPON2) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). Predicted Molecular Mass: 53.1kDa Accurate Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions. Researchers should empirically determine the suitability of the SPON2 spon2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SV HEVFSAPAVP SGTGQTSAEL EVQRRHSLVS FVVRIVPSPD WFVGVDSLDL CDGDRWREQA ALDLYPYDAG TDSGFTFSSP NFATIPQDTV TEITSSSPSH PANSFYYPRL KALPPIARVT LVRLRQSPRA FIPPAPVLPS RDNEIVDSAS VPETPLDCEV SLWSSWGLCG GHCGRLGTKS RTRYVRVQPA NNGSPCPELE EEAECVPDNC V. It is sometimes possible for the material contained within the vial of "Spondin 2 (SPON2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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