B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) Recombinant Protein | Bcl2L recombinant protein

Recombinant B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L)

Cat. Num. AAA2009480

• Gene Names: Bcl2l1; BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L); Recombinant B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L); Bcl2L recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-SQSNRELVV DFLSYKLSQK GYSWSQFSDV EENRTEAPEE TEAERETPSA INGNPSWHLA DSPAVNGATG HSSSLDAREV IPMAAVKQAL REAGDEFELR YRRAFSDLTS QLHITPGTAY QSFEQVVNEL FRDGVNWGRI VAFFSFGGAL CVESVDKEMQ VLVSRIASWM ATYLNDHLEP WIQENGGWDT FVDLYGNNAA AESRKGQERF NR
• Sequence Length: 233

• Applicable Applications for Bcl2L recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser2~Arg212 (Accession # Q64373) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 58037550
• NCBI GeneID: 12048
• NCBI Accession #: NP_033873.3
• NCBI GenBank Nucleotide #: NM_009743.5
• UniProt Accession #: Q64373; O35844; Q60657; Q60658; Q61338
• Molecular Weight: 27.6kDa
• NCBI Official Full Name: bcl-2-like protein 1
• NCBI Official Synonym Full Names: BCL2-like 1
• NCBI Official Symbol: Bcl2l1
• NCBI Official Synonym Symbols: BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
• NCBI Protein Information: bcl-2-like protein 1; apoptosis regulator Bcl-X; B-cell leukemia/lymphoma x; anti-apoptosis regulatory protein
• UniProt Protein Name: Bcl-2-like protein 1
• UniProt Gene Name: Bcl2l1
• UniProt Synonym Gene Names: Bcl2l; Bclx; Bcl2-L-1
• UniProt Entry Name: B2CL1_MOUSE

NCBI Description

This gene encodes a member of the Bcl-2 family of apoptosis regulators. The encoded protein is localized to the inner and outer mitochondrial membranes and regulates the programmed cell death pathway during development and tissue homeostasis. This protein binds to voltage-dependent anion channels in the outer mitochondrial membrane to facilitate the uptake of calcium ions. Mice embryos lacking this gene survived for two weeks and exhibited cell death of immature hematopoietic cells and neurons. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jan 2014]

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Membrane protein, integral; Apoptosis; Mitochondrial; Autophagy

Cellular Component: mitochondrial envelope; centrosome; mitochondrion; integral to membrane; cytosol; mitochondrial outer membrane; cytoskeleton; membrane; mitochondrial inner membrane; mitochondrial membrane; cytoplasm; nucleolus; synapse; intracellular; cytoplasmic vesicle; nucleus; cell junction

Molecular Function: BH domain binding; identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; caspase inhibitor activity; BH3 domain binding; protein kinase binding

Biological Process: positive regulation of apoptosis; apoptosis; cytokinesis; negative regulation of caspase activity; cellular process regulating host cell cycle in response to virus; germ cell development; regulation of apoptosis; response to radiation; regulation of mitochondrial membrane potential; ovarian follicle development; positive regulation of cell proliferation; negative regulation of neuron apoptosis; release of cytochrome c from mitochondria; in utero embryonic development; mitotic cell cycle checkpoint; response to virus; male gonad development; suppression by virus of host apoptosis; cell proliferation; neuron apoptosis; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; response to cycloheximide; spermatogenesis; regulation of mitochondrial membrane permeability; growth; negative regulation of apoptosis