Calpain 1, Large Subunit (CAPN1) Recombinant Protein | CAPN1 recombinant protein

Recombinant Calpain 1, Large Subunit (CAPN1)

Cat. Num. AAA2009456

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Calpain 1; Large Subunit (CAPN1); Recombinant Calpain 1; CAPN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-YPAT FWVNPQFKIR LEEVDDADDY DSRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKI LDTD
• Sequence Length: 713

• Applicable Applications for CAPN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Tyr387~Asp694 (Accession # P97571) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 9506461
• NCBI GeneID: 29153
• NCBI Accession #: NP_062025.1
• NCBI GenBank Nucleotide #: NM_019152.2
• UniProt Accession #: P97571
• Molecular Weight: 37.5kDa
• NCBI Official Full Name: calpain-1 catalytic subunit
• NCBI Official Synonym Full Names: calpain 1, (mu/I) large subunit
• NCBI Official Symbol: Capn1
• NCBI Protein Information: calpain-1 catalytic subunit; CANP 1; muCANP; calpain mu-type; micromolar-calpain; calpain-1 large subunit; calcium-activated neutral proteinase 1
• UniProt Protein Name: Calpain-1 catalytic subunit
• Protein Family: Calpain
• UniProt Gene Name: Capn1
• UniProt Synonym Gene Names: Cls1; CANP 1; muCANP
• UniProt Entry Name: CAN1_RAT

NCBI Description

calcium-dependent protease that may be involved in experimental allergic encephalomyelitis (EAE) [RGD, Feb 2006]

Uniprot Description

CAPN1: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family.

Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.22.52

Cellular Component: focal adhesion; mitochondrion; membrane; lysosome; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: mammary gland involution; protein autoprocessing; receptor catabolic process; proteolysis