Testing Data
Rabbit Calpain 1 Polyclonal Antibody | anti-Capn1 antibody
Calpain 1 Antibody
Reactivity
Human, Mouse, Rat, Bovine, Pig, Rabbit
Applications
Western Blot
Purity
Affinity purified
Synonyms
Calpain 1; Polyclonal Antibody; Calpain 1 Antibody; EC 3.4.22.52; CAPN2; anti-Capn1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
100 ug (0.5 mg/ml) affinity purified rabbit anti-calpain 1 (large subunit) antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Appearance: Colorless liquid
Appearance: Colorless liquid
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
713
Applicable Applications for anti-Capn1 antibody
Western Blot (WB)
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The anti-calpain I antibody recognizes the large subunit (80 kDa) of human Calpain 1. It does not recognize the smaller subunit of human Calpain 1.
Immunogen
Synthetic peptide surrounding amino acid 700 of rat Calpain 1
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for anti-Capn1 antibody
Background: Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,119 Da
NCBI Official Full Name
calpain-1 catalytic subunit
NCBI Official Synonym Full Names
calpain 1, (mu/I) large subunit
NCBI Official Symbol
Capn1
NCBI Protein Information
calpain-1 catalytic subunit
UniProt Protein Name
Calpain-1 catalytic subunit
Protein Family
UniProt Gene Name
Capn1
UniProt Synonym Gene Names
Cls1; CANP 1; muCANP
UniProt Entry Name
CAN1_RAT
NCBI Description
calcium-dependent protease that may be involved in experimental allergic encephalomyelitis (EAE) [RGD, Feb 2006]
Uniprot Description
CAPN1: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family.
Protein type: Motility/polarity/chemotaxis; EC 3.4.22.52; Protease
Cellular Component: focal adhesion; mitochondrion; membrane; lysosome; cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding
Biological Process: mammary gland involution; protein autoprocessing; receptor catabolic process; proteolysis
Research Articles on Capn1
Similar Products
Product Notes
The Capn1 capn1 (Catalog #AAA841284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calpain 1 Antibody reacts with Human, Mouse, Rat, Bovine, Pig, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Calpain 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The anti-calpain I antibody recognizes the large subunit (80 kDa) of human Calpain 1. It does not recognize the smaller subunit of human Calpain 1. Researchers should empirically determine the suitability of the Capn1 capn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calpain 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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