Diamine Oxidase (DAO) Recombinant Protein | DAO recombinant protein

Recombinant Diamine Oxidase (DAO)

Cat. Num. AAA2009381

• Gene Names: Aoc1; Abp; Abp1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Diamine Oxidase (DAO); Recombinant Diamine Oxidase (DAO); DAO recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-GCDDRCLTFT DVAPRGVASG QRRSWFIVQR YVEGYFLHPT GLEILLDHGS TDVQDWRVEQ LWYNGKFYNN PEELARKYAV GEVDTVVLED PLPNGTEKPP LFSSYKPRGE FHTPVNVAGP HVVQPSGPRY KLEGNTVLYG GWSFSYRLRS SSG
• Sequence Length: 746

• Applicable Applications for DAO recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gly176~Gly328 (Accession # P36633) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 543747
• NCBI GeneID: 65029
• UniProt Accession #: P36633; Q63973
• Molecular Weight: 18.5kDa
• NCBI Official Full Name: Amiloride-sensitive amine oxidase
• NCBI Official Synonym Full Names: amine oxidase, copper containing 1
• NCBI Official Symbol: Aoc1
• NCBI Official Synonym Symbols: Abp; Abp1
• NCBI Protein Information: amiloride-sensitive amine oxidase [copper-containing]; amiloride-sensitive amine oxidase [copper-containing]; DAO; histaminase; diamine oxidase; amiloride-binding protein 1; amine oxidase copper domain-containing protein 1; amiloride binding protein 1 (amine oxidase, copper-containing)
• UniProt Protein Name: Amiloride-sensitive amine oxidase [copper-containing]
• Protein Family: D-amino-acid oxidase
• UniProt Gene Name: Aoc1
• UniProt Synonym Gene Names: Abp1; DAO
• UniProt Entry Name: AOC1_RAT

NCBI Description

binds amiloride and some of its derivatives; may form an epithelial-specific Na+ channel [RGD, Feb 2006]

Uniprot Description

ABP1: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. Belongs to the copper/topaquinone oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Oxidoreductase; EC 1.4.3.22; Secreted; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - histidine; Secreted, signal peptide

Cellular Component: extracellular space; tight junction; plasma membrane

Molecular Function: amine oxidase activity; heparin binding; protein homodimerization activity; copper ion binding; zinc ion binding; protein complex binding; receptor activity; drug binding; cation channel activity; calcium ion binding; quinone binding

Biological Process: response to drug; response to antibiotic; amine metabolic process; cation transport