Galactosylceramidase (GALC) Recombinant Protein | GALC recombinant protein

Recombinant Galactosylceramidase (GALC)

Cat. Num. AAA2009335

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Galactosylceramidase (GALC); Recombinant Galactosylceramidase (GALC); GALC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-EFD GIGAVSGGGA TSRLLVNYPE PYRSEILDYL FKPNFGASLH ILKVEIGGDG QTTDGTEPSH MHYELDENYF RGYEWWLMKE AKKRNPNIIL MGLPWSFPGW LGKGFSWPYV NLQLTA
• Sequence Length: 668

• Applicable Applications for GALC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Glu38~Ala156 (Accession # G3V6H1) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 54312098
• NCBI GeneID: 314360
• NCBI Accession #: NP_001005888.1
• NCBI GenBank Nucleotide #: NM_001005888.1
• UniProt Accession #: G3V6H1; Q5YKG1
• Molecular Weight: 15.0kDa
• NCBI Official Full Name: galactocerebrosidase
• NCBI Official Synonym Full Names: galactosylceramidase
• NCBI Official Symbol: Galc
• NCBI Protein Information: galactocerebrosidase
• UniProt Protein Name: Galactosylceramidase
• Protein Family: Galactocerebrosidase
• UniProt Gene Name: Galc
• UniProt Entry Name: G3V6H1_RAT

Uniprot Description

GALC: Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Defects in GALC are the cause of leukodystrophy globoid cell (GLD); also known as Krabbe disease. This autosomal recessive disorder results in the insufficient catabolism of several galactolipids that are important in the production of normal myelin. Clinically, the most frequent form is the infantile form. Most patients (90%) present before six months of age with irritability, spasticity, arrest of motor and mental development, and bouts of temperature elevation without infection. This is followed by myoclonic jerks of arms and legs, oposthotonus, hypertonic fits, and mental regression, which progresses to a severe decerebrate condition with no voluntary movements and death from respiratory infections or cerebral hyperpyrexia before 2 years of age. However, a significant number of cases with later onset, presenting with unexplained blindness, weakness and/or progressive motor, and sensory neuropathy that can progress to severe mental incapacity and death, have been identified. Belongs to the glycosyl hydrolase 59 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.2.1.46; Lipid Metabolism - sphingolipid

Cellular Component: mitochondrion

Molecular Function: galactosylceramidase activity

Biological Process: carbohydrate metabolic process; galactosylceramide catabolic process