Deiodinase, Iodothyronine, Type III (DIO3) Recombinant Protein | DIO3 recombinant protein

Recombinant Deiodinase, Iodothyronine, Type III (DIO3)

Cat. Num. AAA2009300

• Gene Names: Dio3; 5DIII; DIOIII
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Deiodinase; Iodothyronine; Type III (DIO3); Recombinant Deiodinase; DIO3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and MBP-tag, its sequence is listed below.; MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGSEF- RLCAQTASCL VLFPRFLGTA FMLWLLDFLC IRKHFLRRRH PDHPEPEVEL NSEGEEMPPD DPPICVSDDN RLCTLASLKA VWHGQKLDFF KQAHEGGPAP NSEVVRPDGF QSQRILDYAQ GTRPLVLNFG SCTUPPFMAR MSAFQRLVTK YQRDVDFLII YIEEAHPSDG WVTTDSPYVI PQHRSLEDRV SAARVLQQGA PGCALVLDTM ANSSSSAYGA YFERLYVIQS GTIMYQGGRG PDGYQVSELR TWLERYDEQL HGTRPRR
• Sequence Length: 304

• Applicable Applications for DIO3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Arg11~Arg277 (Accession # P49897) with two N-terminal Tags, His-tag and MBP-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 255982585
• NCBI GeneID: 29475
• NCBI Accession #: NP_058906.3
• NCBI GenBank Nucleotide #: NM_017210.3
• UniProt Accession #: P49897
• Molecular Weight: 77.0kDa
• NCBI Official Full Name: type III iodothyronine deiodinase
• NCBI Official Synonym Full Names: deiodinase, iodothyronine, type III
• NCBI Official Symbol: Dio3
• NCBI Official Synonym Symbols: 5DIII; DIOIII
• NCBI Protein Information: type III iodothyronine deiodinase; type 3 DI; type-III 5'-deiodinase; deiodinase, iodothyronine, type 3
• UniProt Protein Name: Type III iodothyronine deiodinase
• Protein Family: Thyroxine 5-deiodinase
• UniProt Gene Name: Dio3
• UniProt Synonym Gene Names: Itdi3; Txdi3
• UniProt Entry Name: IOD3_RAT

NCBI Description

The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. It contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family.

Protein type: Oxidoreductase; Membrane protein, integral; EC 1.97.1.11

Cellular Component: integral to membrane; plasma membrane; endosome membrane

Molecular Function: thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity

Biological Process: thyroid hormone catabolic process; response to hypoxia; hormone biosynthetic process; positive regulation of multicellular organism growth; thyroid hormone metabolic process