Neuropilin 1 (NRP1) Recombinant Protein | NRP1 recombinant protein

Recombinant Neuropilin 1 (NRP1)

Cat. Num. AAA2009184

• Gene Names: Nrp1; Nrp
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Neuropilin 1 (NRP1); Recombinant Neuropilin 1 (NRP1); NRP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and MBP-tag, its sequence is listed below.; MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGSEF- CGGT IKIENPGYLT SPGYPHSYHP SEKCEWLIQA PEPYQRIMIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW GKFCGKIAPS PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN YTCGGT IKIENPGYLT SPGYPHSYHP SEKCEWLIQA PEPYQRIMIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW GKFCGKIAPS PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN YT
• Sequence Length: 922

• Applicable Applications for NRP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Cys27~Thr152 (Accession # Q9QWJ9) with two N-terminal Tags, His-tag and MBP-tag
• Subcellular Location: Membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 26023947
• NCBI GeneID: 246331
• NCBI Accession #: NP_659566.1
• NCBI GenBank Nucleotide #: NM_145098.2
• UniProt Accession #: Q9QWJ9
• Molecular Weight: 75.4kDa
• NCBI Official Full Name: neuropilin-1
• NCBI Official Synonym Full Names: neuropilin 1
• NCBI Official Symbol: Nrp1
• NCBI Official Synonym Symbols: Nrp
• NCBI Protein Information: neuropilin-1; vascular endothelial cell growth factor 165 receptor
• UniProt Protein Name: Neuropilin-1
• Protein Family: Neuropilin
• UniProt Gene Name: Nrp1
• UniProt Entry Name: NRP1_RAT

NCBI Description

transmembrane protein that acts as a receptor for semaphorin III; may play a role in neuronal development [RGD, Feb 2006]

Uniprot Description

NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: extracellular space; cell surface; growth cone; focal adhesion; cell soma; axon; early endosome; integral to membrane; neurofilament; plasma membrane; cytosol

Molecular Function: heparin binding; vascular endothelial growth factor receptor activity; protein binding; semaphorin receptor activity; growth factor binding; metal ion binding

Biological Process: facial nerve structural organization; axon guidance; peptidyl-tyrosine phosphorylation; heart development; neuron migration; platelet-derived growth factor receptor signaling pathway; vestibulocochlear nerve structural organization; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; trigeminal nerve morphogenesis; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; positive chemotaxis; branchiomotor neuron axon guidance; response to wounding; dendrite development; angiogenesis; negative regulation of neuron apoptosis; trigeminal nerve structural organization; nervous system development; cell migration; axonal fasciculation; hepatocyte growth factor receptor signaling pathway; axon extension involved in axon guidance; patterning of blood vessels; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; otic placode formation; regulation of axon extension involved in axon guidance; positive regulation of axon extension involved in axon guidance; artery morphogenesis; neuron development; nerve development; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; retinal ganglion cell axon guidance